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In Solidago canadensis the biosynthesis results in the pro-R hydrogen at C-1 of the farnesy diphosphate ending up at C-11 of the (-)-germacrene D. With Streptomyces coelicolor the pro-S hydrogen at C-1 ends up at C-11 of the (-)-germacrene D. Group: Enzymes. Enzyme Commission Number: EC 4.2.3.75. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5237; (-)-germacrene D synthase; EC 4.2.3.75. Cat No: EXWM-5237.
(+)-germacrene D synthase
Requires Mg2+, Mn2+, Ni2+ or Co2+. The formation of (+)-germacrene D involves a 1,2-hydride shift whereas for (-)-germacrene D there is a 1,3-hydride shift (see EC 4.2.3.75). Group: Enzymes. Enzyme Commission Number: EC 4.2.3.77. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5239; (+)-germacrene D synthase; EC 4.2.3.77. Cat No: EXWM-5239.
cubebol synthase
Requires Mg2+. The enzyme gives 28% cubebol, 29% (-)-germacrene D, 10% (+)-Δ-cadinene and traces of several other sesquiterpenoids. See also EC 4.2.3.75 (-)-germacrene D synthase and EC 4.2.3.13 (+)-Δ-cadinene synthase. Group: Enzymes. Synonyms: Cop4. Enzyme Commission Number: EC 4.2.3.91. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5255; cubebol synthase; EC 4.2.3.91; Cop4. Cat No: EXWM-5255.
(E,E)-germacrene B synthase
(E,E)-germacrene B synthase (EC 4.2.3.71) is an enzyme with systematic name (2E,6E)-farnesyl-diphosphate diphosphate-lyase ((E,E)-germacrene-B-forming). Group: Enzymes. Enzyme Commission Number: EC 4.2.3.71. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5233; (E,E)-germacrene B synthase; EC 4.2.3.71. Cat No: EXWM-5233.
germacradienol synthase
Requires Mg2+ for activity. H-1si of farnesyl diphosphate is lost in the formation of (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol. Formation of (-)-germacrene D involves a stereospecific 1,3-hydride shift of H-1si of farnesyl diphosphate. Both products are formed from a common intermediate. Other enzymes produce germacrene D as the sole product using a different mechanism. The enzyme mediates a key step in the biosynthesis of geosmin (see EC 4.1.99.16 geosmin synthase), a widely occurring metabolite of many streptomycetes, bacteria and fungi. Also catalyses the reaction of EC 4.2.3.75, (-)-germacrene D synthase. Group: Enzymes. Synonyms: germacradienol/germacrene-D synthase; 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase [(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol-forming]. Enzyme Commission Number: EC 4.2.3.22. CAS No. 211049-88-6. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5179; germacradienol synthase; EC 4.2.3.22; 211049-88-6; germacradienol/germacrene-D synthase; 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase [(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol-forming]. Cat No: EXWM-5179.
germacrene-A synthase
Requires Mg2+ for activity. While germacrene A is an enzyme-bound intermediate in the biosynthesis of a number of phytoalexins, e.g. EC 4.2.3.9 (aristolochene synthase) from some species and EC 4.2.3.21 (vetispiradiene synthase), it is the sole sesquiterpenoid product formed in chicory. Group: Enzymes. Synonyms: germacrene A synthase; (+)-germacrene A synthase; (+)-(10R)-germacrene A synthase; GAS; 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase (germacrene-A-forming). Enzyme Commission Number: EC 4.2.3.23. CAS No. 213763-55-4. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5180; germacrene-A synthase; EC 4.2.3.23; 213763-55-4; germacrene A synthase; (+)-germacrene A synthase; (+)-(10R)-germacrene A synthase; GAS; 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase (germacrene-A-forming). Cat No: EXWM-5180.
kunzeaol synthase
Isolated from the root of the plant Thapsia garganica. The enzyme also produces germacrene D, bicyclogermacrene and traces of other sesquiterpenoids. See EC 4.2.3.77, (+)-germacrene D synthase and EC 4.2.3.100, bicyclogermacrene synthase. Group: Enzymes. Synonyms: TgTPS2 (gene name). Enzyme Commission Number: EC 4.2.3.143. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5157; kunzeaol synthase; EC 4.2.3.143; TgTPS2 (gene name). Cat No: EXWM-5157.
aristolochene synthase
The initial internal cyclization produces the monocyclic intermediate germacrene A; further cyclization and methyl transfer converts the intermediate into aristolochene. While in some species germacrene A remains as an enzyme-bound intermediate, it has been shown to be a minor product of the reaction in Penicillium roqueforti (see also EC 4.2.3.23, germacrene-A synthase). The enzyme from Penicillium roqueforti requires Mg2+. Mn2+ can partially substitute, at low concentrations. Aristolochene is the likely parent compound for a number of sesquiterpenes produced by filamentous fungi. Group: Enzymes. Synonyms: sesquiterpene cyclase; trans,trans-farnesyl diphosphate aristolochene-lyase; trans,trans-farnesyl-diphosphate diphosphate-lyase (cyclizing, aristolochene-forming). Enzyme Commission Number: EC 4.2.3.9. CAS No. 94185-89-4. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5253; aristolochene synthase; EC 4.2.3.9; 94185-89-4; sesquiterpene cyclase; trans,trans-farnesyl diphosphate aristolochene-lyase; trans,trans-farnesyl-diphosphate diphosphate-lyase (cyclizing, aristolochene-forming). Cat No: EXWM-5253.
avermitilol synthase
Requires Mg2+. The recombinent enzyme gives avermitilol (85%) plus traces of germacrene A, germacrene B and viridiflorol. The (1S)-hydrogen of farnesyl diphosphate is retained. Group: Enzymes. Enzyme Commission Number: EC 4.2.3.96. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5260; avermitilol synthase; EC 4.2.3.96. Cat No: EXWM-5260.
germacrene C synthase
Germacrene C synthase (EC 4.2.3.60) is an enzyme with systematic name (2E,6E)-farnesyl-diphosphate diphosphate-lyase (germacrene-C-forming). Group: Enzymes. Enzyme Commission Number: EC 4.2.3.60. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5221; germacrene C synthase; EC 4.2.3.60. Cat No: EXWM-5221.
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