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| Product | Description | |
|---|---|---|
| 5-Bromo-4-chloro-3-indolyl-B-D-galactopyranoside | Bromo-4-chloro-3-indolyl-B-D-galactopyranoside. CAS No. 7240-90-6. Categories: x-gal. |  Pennsylvania PA |
| X-Gal | X-Gal is a fluorecent substrate for β-galactosidase and blue-white selection of recombinant bacterial colonies with the lac+ genotype. Synonyms: 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside; X-b-D-Galactoside; BCIG. CAS No. 7240-90-6. Molecular formula: C14H15BrClNO6. Mole weight: 408.64. |  |
| X-GAL | X-GAL (BCIG) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity [1] [2]. Uses: Scientific research. Group: Fluorescent dye. Alternative Names: BCIG. CAS No. 7240-90-6. Pack Sizes: 200 mg; 500 mg; 1 g; 2 g. Product ID: HY-15934. |  |
| X-Gal, 40mg/ml in DMF (5-Bromo-4-chloro-3-indolyl-b-D-galactoside galactopyranoside, Xgal) | Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white. Group: Biochemicals. Alternative Names: 5-Bromo-4-chloro-3-indolyl-ß-D-galactoside (galactopyranoside) in Dimethylformamide. Grades: Molecular Biology Grade. CAS No. 7240-90-6,68-12-2. Pack Sizes: 10ml, 5x10ml. Molecular Formula: C14H15BrClNO6 DMF, Molecular Weight: 408.61. US Biological Life Sciences. |  Worldwide |
| X-Gal (5-Bromo-4-chloro-3-indolyl-b-D-galactoside galactopyranoside, Xgal) | Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white.Gene Cloning:In gene cloning, X-gal is used as a visual indication of whether a cell expresses a functional β-galactosidase enzyme in a technique called blue/white screening. This method of screening is a convenient way of distinguishing a successful cloning product from other unsuccessful ones.The blue/white screening method relies on the principle of α-complementation of the β-galactosidase gene, where a fragment of the lacZ gene (lacZα) in the plasmid can complement another mutant lacZ gene (lacZΔM15) in the cell. Both genes by themselves produce non-functional peptides, however, when expressed together, as when a plasmid containing lacZα is transformed into a lacZΔM15 cells, they form a functional β-galactosidase. The presence of an active β-galactosidase may be detected when cells are grown in plates containing X-gal, the blue-colored product precipitated within cells resulted in the characteristic blue colonies. However, the multiple cloning site, where a gene of interest may be ligated into the plasmid vector, is located within the lacZα gene.... . Group: Biochemicals. Alternative Names: X-Gal, 5-Bromo-4-chloro-3-indolyl-b-D-galactoside (galactopyranoside), BCIG. Grades: Molecular Biology Grade. CAS No. 7240-90-6. Pack Sizes: 100mg, 250mg, 1g, 10g. Molecular Formula: C14H15BrClNO6, Molecular Weight: 408.61. US Biological Life Sciences. | Worldwide |
| X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) | 100mg Pack Size. Group: Stains & Indicators. Formula: C14H15BrClNO6. CAS No. 7240-90-6. Prepack ID 11322533-100mg. Molecular Weight 408.63. See USA prepack pricing. |  |
| X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) | 1g Pack Size. Group: Stains & Indicators. Formula: C14H15BrClNO6. CAS No. 7240-90-6. Prepack ID 11322533-1g. Molecular Weight 408.63. See USA prepack pricing. | |
| X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) | 5g Pack Size. Group: Stains & Indicators. Formula: C14H15BrClNO6. CAS No. 7240-90-6. Prepack ID 11322533-5g. Molecular Weight 408.63. See USA prepack pricing. | |
| X-Gal (aka X-b-D-galactoside) | X-Gal (aka X-b-D-galactoside). CAS No. 7240-90-6. Categories: x-gal. | Pennsylvania PA |
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