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This cytosolic sesquiterpenoid synthase requires a divalent cation cofactor (Mg2+ or, to a lesser extent, Mn2+) to neutralize the negative charge of the diphosphate leaving group. While unlikely to encounter geranyl diphosphate (GDP) in vivo as it is localized to plastids, the enzyme can use GDP as a substrate in vitro to produce (+)-(4R)-limonene [cf. EC 4.2.3.20, (R)-limonene synthase]. The enzyme is induced as part of a defense mechanism in the grand fir Abies grandis as a response to stem wounding. Group: Enzymes. Synonyms: bisabolene synthase. Enzyme Commission Number: EC 4.2.3.38. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5196; α-bisabolene synthase; EC 4.2.3.38; bisabolene synthase. Cat No: EXWM-5196.
(E)-γ-bisabolene synthase
(E)-gamma-bisabolene synthase (EC 4.2.3.59) is an enzyme with systematic name (2E,6E)-farnesyl-diphosphate diphosphate-lyase ((E)-gamma-bisabolene-forming). Group: Enzymes. Enzyme Commission Number: EC 4.2.3.59. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5219; (E)-γ-bisabolene synthase; EC 4.2.3.59. Cat No: EXWM-5219.
(S)-β-bisabolene synthase
The synthesis of (S)-β-macrocarpene from (2E,6E)-farnesyl diphosphate proceeds in two steps. The first step is the cyclization to (S)-β-bisabolene. The second step is the isomerization to (S)-β-macrocarpene (cf. EC 5.5.1.17, (S)-β-macrocarpene synthase). The enzyme requires Mg2+ or Mn2+ for activity. Group: Enzymes. Enzyme Commission Number: EC 4.2.3.55. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5215; (S)-β-bisabolene synthase; EC 4.2.3.55. Cat No: EXWM-5215.
(Z)-γ-bisabolene synthase
This sesquiterpenoid enzyme is constitutively expressed in the root, hydathodes and stigma of the plant Arabidopsis thaliana. If the leaves of the plant are wounded, e.g. by cutting, the enzyme is also induced close to the wound site. The sesquiterpenoids (E)-nerolidol and α-bisabolol are also produced by this enzyme as minor products. Group: Enzymes. Enzyme Commission Number: EC 4.2.3.40. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5199; (Z)-γ-bisabolene synthase; EC 4.2.3.40. Cat No: EXWM-5199.
7-epi-sesquithujene synthase
The enzyme from Zea mays, variety B73, gives mainly 7-epi-sesquithujene with (S)-β-bisabolene and traces of other sesquiterpenoids, cf. EC 4.2.3.55 (S)-β-bisabolene synthase. It requires Mg2+ or Mn2+. The product ratio is dependent on which metal ion is present. 7-epi-Sesquithujene is an attractant for the emerald ash borer beetle. Group: Enzymes. Synonyms: TPS4-B73. Enzyme Commission Number: EC 4.2.3.101. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5112; 7-epi-sesquithujene synthase; EC 4.2.3.101; TPS4-B73. Cat No: EXWM-5112.
epi-cedrol synthase
The enzyme is activated by Mg2+. Similar to many other plant terpenoid synthases, this enzyme produces many products from a single substrate. The predominant product is the cyclic sesquiterpenoid alcohol, 8-epi-cedrol, with minor products including cedrol and the olefins α-cedrene, β-cedrene, (E)-β-farnesene and (E)-α-bisabolene. Group: Enzymes. Synonyms: 8-epicedrol synthase; epicedrol synthase. Enzyme Commission Number: EC 4.2.3.39. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5197; epi-cedrol synthase; EC 4.2.3.39; 8-epicedrol synthase; epicedrol synthase. Cat No: EXWM-5197.
exo-α-bergamotene synthase
The enzyme synthesizes a mixture of sesquiterpenoids from (2E,6E)-farnesyl diphosphate. As well as (-)-exo-α-bergamotene (74%) there were (E)-nerolidol (10%), (Z)-α-bisabolene (6%), (E)-β-farnesene (5%) and β-sesquiphellandrene (1%). Group: Enzymes. Synonyms: trans-α-bergamotene synthase; LaBERS (gene name). Enzyme Commission Number: EC 4.2.3.81. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5244; exo-α-bergamotene synthase; EC 4.2.3.81; trans-α-bergamotene synthase; LaBERS (gene name). Cat No: EXWM-5244.
(S)-β-macrocarpene synthase
The synthesis of (S)-β-macrocarpene from (2E,6E)-farnesyl diphosphate proceeds in two steps. The first step is the cyclization to (S)-β-bisabolene (cf. EC 4.2.3.55, (S)-β-bisabolene synthase). The second step is the isomerization to (S)-β-macrocarpene. Group: Enzymes. Synonyms: TPS6; TPS11; (S)-β-macrocarpene lyase (decyclizing). Enzyme Commission Number: EC 5.5.1.17. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5618; (S)-β-macrocarpene synthase; EC 5.5.1.17; TPS6; TPS11; (S)-β-macrocarpene lyase (decyclizing). Cat No: EXWM-5618.
sesquithujene synthase
The enzyme from Zea mays, variety Delprim, gives mainly sesquithujene with (S)-β-bisabolene and (E)-β-farnesene plus traces of other sesquiterpenoids, cf. EC 4.2.3.55 [(S)-β-bisabolene synthase] and EC 4.2.3.47 (β-farnesene synthase). It requires Mg2+ or Mn2+. The exact product ratio is dependent on which metal ion is present. Group: Enzymes. Synonyms: TPS5-Del1. Enzyme Commission Number: EC 4.2.3.102. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5113; sesquithujene synthase; EC 4.2.3.102; TPS5-Del1. Cat No: EXWM-5113.
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