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| Product | Description | |
|---|---|---|
| cDNA Library | cDNA Library Construction Service can Include: Group: Molecular Biology. US Biological Life Sciences. |  Worldwide |
| cDNA Library, Vector Preparation | cDNA Library Construction Service Includes: Group: Molecular Biology. US Biological Life Sciences. | Worldwide |
| Aminoallyl-UTP - DYQ-661 | Aminoallyl-UTP, a potent and versatile chemical reagent in the realm of biomedical research, is employed in the detection and labeling of RNA molecules. It plays a pivotal role in the analysis of microarrays and the synthesis of cDNA libraries. When used in tandem with reverse transcriptase, it is highly efficacious in labeling RNA for the purposes of Northern blotting and in situ hybridization assays. Synonyms: 5-(3-Aminoallyl)-uridine-5'-triphosphate, labeled with DYQ 661, Triethylammonium salt. Grades: ≥ 95% by HPLC. Molecular formula: C51H61N5O23P3S2 (Anion). Mole weight: 1269.10 (Anion). |  |
| Reverse Transcriptase from Moloney Murine Leukemia Virus, Recombinant | This Reverse Transcriptase has exceptionally strong strand displacement activity and enables efficient preparation of cDNA up to 12 kb in length. It is robust, versatile and well-suited for applications requiring full-length cDNA such as preparation of cDNA libraries and other techniques involving first strand cDNA synthesis (RT-PCR, preparation of cDNA probes, real-time quantitative RT-PCR). It can be used for performing a reverse transcription reaction with any RNA template including GC-rich templates and RNAs with high levels of secondary structure. This enzyme is a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase and is v...ptases tested; 4. highly specific: low rates of non-specific annealing, even on incompletely denatured rna; 5. works on challenging templates: excellent results even with gc-rich templates and templates with high levels of secondary structure 6. highly sensitive: use less of your precious rna samples; 7. demonstrated success with reverse transcription reaction times of 30 min. (60 min. for longer transcripts). Applications: Rt-pcr; first strand cdna synthesis; cdna probe preparation; synthesis of cdna libraries with a high proportion of full-length cdnas. Group: Enzymes. Synonyms: Reverse transcriptase; RT. RT. Storage: Storage at-20°C. Source: E. coli. Spec |  |
| Ribonuclease R from E. coli | RNase R is an 3'-->5' exoribonuclease closely related to RNase II, which has been shown to be involved in selective mRNA degradation, particularly of non stop mRNAs in bacteria. RNase R has homologues in many other organisms. When a part of another larger protein has a domain that is very similar to RNase R, this is called an RNase R domain. Rnase r is provided as a 250 u size (20 u/μl; 1 μg/μl) and is supplied with a 10x rnase r reaction buffer. Applications: O alternative splicing studieso gene expression studieso intron cdna productiono intronic screening of cdna librarieso isolation of splicing intermediates and lariats. Group: Enzymes. Synonyms: RNase R; Ribonuclease. Enzyme Commission Number: EC 3.1.13.1. Activity: 20 U/μl. Storage: Store only at -20°C in a freezer without a defrost cycle. Source: E. coli. RNase R; Ribonuclease; Ribonuclease R. Cat No: NATE-1636. | |
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