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The enzyme can be dissociated into components, two of which are identical with EC 2.8.3.11 (citramalate CoA-transferase) and EC 4.1.3.25 (citramalyl-CoA lyase). Group: Enzymes. Synonyms: citramalate pyruvate-lyase; citramalate synthase; citramalic-condensing enzyme; citramalate synthetase; citramalic synthase; (S)-citramalate lyase; (+)-citramalate pyruvate-lyase; citramalate pyruvate lyase; (3S)-citramalate pyruvate-lyase; (2S)-2-hydroxy-2-methylbutanedioate pyruvate-lyase. Enzyme Commission Number: EC 4.1.3.22. CAS No. 9027-93-4. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-4903; citramalate lyase; EC 4.1.3.22; 9027-93-4; citramalate pyruvate-lyase; citramalate synthase; citramalic-condensing enzyme; citramalate synthetase; citramalic synthase; (S)-citramalate lyase; (+)-citramalate pyruvate-lyase; citramalate pyruvate lyase; (3S)-citramalate pyruvate-lyase; (2S)-2-hydroxy-2-methylbutanedioate pyruvate-lyase. Cat No: EXWM-4903.
(S)-2-methylmalate dehydratase
Also hydrates fumarate to (S)-malate. Group: Enzymes. Synonyms: mesaconate hydratase; (+)-citramalate hydro-lyase; L-citramalate hydrolase; citramalate dehydratase; (+)-citramalic hydro-lyase; mesaconate mesaconase; mesaconase; (S)-2-methylmalate hydro-lyase. Enzyme Commission Number: EC 4.2.1.34. CAS No. 9027-94-5. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5025; (S)-2-methylmalate dehydratase; EC 4.2.1.34; 9027-94-5; mesaconate hydratase; (+)-citramalate hydro-lyase; L-citramalate hydrolase; citramalate dehydratase; (+)-citramalic hydro-lyase; mesaconate mesaconase; mesaconase; (S)-2-methylmalate hydro-lyase. Cat No: EXWM-5025.
citramalate CoA-transferase
The enzyme is a component of EC 4.1.3.22 citramalate lyase. Also catalyses the transfer of thioacyl carrier protein from its acetyl thioester to citramalate. Group: Enzymes. Enzyme Commission Number: EC 2.8.3.11. CAS No. 9033-60-7. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-3406; citramalate CoA-transferase; EC 2.8.3.11; 9033-60-7. Cat No: EXWM-3406.
itaconyl-CoA hydratase
This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. This enzyme participates in c5-branched dibasic acid metabolism. Group: Enzymes. Synonyms: itaconyl coenzyme A hydratase; citramalyl-CoA hydro-lyase. Enzyme Commission Number: EC 4.2.1.56. CAS No. 37290-83-8. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5045; itaconyl-CoA hydratase; EC 4.2.1.56; 37290-83-8; itaconyl coenzyme A hydratase; citramalyl-CoA hydro-lyase. Cat No: EXWM-5045.
malyl-CoA lyase
The enzyme from the bacterium Chloroflexus aurantiacus, which participates in the 3-hydroxypropanoate cycle for carbon assimilation, also has the activity of EC 4.1.3.25, (3S)-citramalyl-CoA lyase. The enzymes from Rhodobacter species are part of acetate assimilation pathways. The reactions are reversible. Group: Enzymes. Synonyms: malyl-coenzyme A lyase; (3S)-3-carboxy-3-hydroxypropanoyl-CoA glyoxylate-lyase; mclA (gene name); mcl1 (gene name); (3S)-3-carboxy-3-hydroxypropanoyl-CoA glyoxylate-lyase (acetyl-CoA-forming); L-malyl-CoA lyase. Enzyme Commission Number: EC 4.1.3.24. CAS No. 37290-67-8. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-4904; malyl-CoA lyase; EC 4.1.3.24; 37290-67-8; malyl-coenzyme A lyase; (3S)-3-carboxy-3-hydroxypropanoyl-CoA glyoxylate-lyase; mclA (gene name); mcl1 (gene name); (3S)-3-carboxy-3-hydroxypropanoyl-CoA glyoxylate-lyase (acetyl-CoA-forming); L-malyl-CoA lyase. Cat No: EXWM-4904.
(±)-Potassium citramalate monohydrate
Heterocyclic Organic Compound. Alternative Names: (±)-PotassiuM citraMalate Monohydrate;2-Hydroxy-2-methylbutanedioic acid dipotassium salt. CAS No. 1030365-02-6. Molecular formula: C5H6O5K2. Catalog: ACM1030365026.
(R)-2-methylmalate dehydratase
Requires Fe2+. Group: Enzymes. Synonyms: citraconate hydratase; citraconase; citramalate hydro-lyase; (-)-citramalate hydro-lyase; (R)-2-methylmalate hydro-lyase. Enzyme Commission Number: EC 4.2.1.35. CAS No. 9027-92-3. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5026; (R)-2-methylmalate dehydratase; EC 4.2.1.35; 9027-92-3; citraconate hydratase; citraconase; citramalate hydro-lyase; (-)-citramalate hydro-lyase; (R)-2-methylmalate hydro-lyase. Cat No: EXWM-5026.
(R)-citramalate synthase
One of the enzymes involved in a novel pyruvate pathway for isoleucine biosynthesis that is found in some, mainly archaeal, bacteria. The enzyme can be inhibited by isoleucine, the end-product of the pathway, but not by leucine. The enzyme is highly specific for pyruvate as substrate, as the 2-oxo acids 3-methyl-2-oxobutanoate, 2-oxobutanoate, 4-methyl-2-oxopentanoate, 2-oxohexanoate and 2-oxoglutarate cannot act as substrate. Group: Enzymes. Synonyms: CimA. Enzyme Commission Number: EC 2.3.1.182. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-2124; (R)-citramalate synthase; EC 2.3.1.182; CimA. Cat No: EXWM-2124.
(R)-citramalyl-CoA lyase
Requires Mn2+ ions for activity. The enzyme, purified from the bacterium Chloroflexus aurantiacus, has no activity with (3S)-citramalyl-CoA (cf. EC 4.1.3.25, (S)-citramalyl-CoA lyase). Group: Enzymes. Synonyms: Ccl. Enzyme Commission Number: EC 4.1.3.46. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-4923; (R)-citramalyl-CoA lyase; EC 4.1.3.46; Ccl. Cat No: EXWM-4923.
(S)-citramalyl-CoA lyase
Requires Mg2+ ions for activity. The enzyme from the bacterium Clostridium tetanomorphum is a component of EC 4.1.3.22, citramalate lyase. It also acts on (3S)-citramalyl thioacyl-carrier protein. The enzyme from the bacterium Chloroflexus aurantiacus also has the activity of EC 4.1.3.24, malyl-CoA lyase. It has no activity with (3R)-citramalyl-CoA (cf. EC 4.1.3.46, (R)-citramalyl-CoA lyase). Group: Enzymes. Synonyms: citramalyl coenzyme A lyase (ambiguous); (+)-CMA-CoA lyase; (3S)-citramalyl-CoA pyruvate-lyase; Mcl (ambiguous); citramalyl-CoA lyase (ambiguous). Enzyme Commission Number: EC 4.1.3.25. CAS No. 37290-68-9. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-4905; (S)-citramalyl-CoA lyase; EC 4.1.3.25; 37290-68-9; citramalyl coenzyme A lyase (ambiguous); (+)-CMA-CoA lyase; (3S)-citramalyl-CoA pyruvate-lyase; Mcl (ambiguous); citramalyl-CoA lyase (ambiguous). Cat No: EXWM-4905.
succinyl-CoA-D-citramalate CoA-transferase
The enzyme, purified from the bacterium Clostridium tetanomorphum, can also accept itaconate as acceptor, with lower efficiency. Group: Enzymes. Synonyms: Sct. Enzyme Commission Number: EC 2.8.3.20. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-3416; succinyl-CoA-D-citramalate CoA-transferase; EC 2.8.3.20; Sct. Cat No: EXWM-3416.
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