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Dihydroethidium Dihydroethidium is a cell-permeable blue fluorescent dye, which intercalates with nucleic acids and emits a red fluorescence detectable qualitatively by fluorescent microscopy or quantitatively by HPLC. It is a superoxide indicator. It exhibits blue fluorescence in the cytosol until oxidizedto ethidium, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red. It is neuroprotective by reducing superoxide in mice after stroke. It has been used to detect reactive oxygen species during the phagocytic respiratory burst and for the detection of intracellular superoxide in cultured cells. Synonyms: Hydroethidine; PD-MY 003; 5-ethyl-5,6-dihydro-6-phenyl-3,8-phenanthridinediamine; 5-Ethyl-6-phenyl-6H-phenanthridine-3,8-diamine. Grades: ≥98%. CAS No. 104821-25-2. Molecular formula: C21H21N3. Mole weight: 315.4. BOC Sciences 9
Dihydroethidium Other Fluorophores. Alternative Names: 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenthridine,3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenthridine. CAS No. 104821-25-2. Molecular formula: C21H21N3. Mole weight: 315.41. Appearance: Pink solid. Purity: 95%+. IUPACName: 5-ethyl-6-phenyl-6H-phenanthridine-3,8-diamine. Canonical SMILES: CCN1C (C2=C (C=CC (=C2)N)C3=C1C=C (C=C3)N)C4=CC=CC=C4. Catalog: ACM104821252-1. Alfa Chemistry. 5

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