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This protein catalyses the successive addition of two glutamate residues to cofactor F420 by two distinct and independent reactions. In the reaction described here the enzyme attaches a glutamate via its α-amine group to F420-0. In the second reaction (EC 6.3.2.34, coenzymeF420-1-γ-L-glutamate ligase) it catalyses the addition of a second L-glutamate residue to the γ-carboxyl of the first glutamate. Group: Enzymes. Synonyms: CofE-AF; MJ0768; CofE. Enzyme Commission Number: EC 6.3.2.31. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5748; coenzymeF420-0:L-glutamate ligase; EC 6.3.2.31; CofE-AF; MJ0768; CofE. Cat No: EXWM-5748.
coenzymeF420-1:γ-L-glutamate ligase
This protein catalyses the successive addition of two glutamate residues to cofactor F420 by two distinct and independent reactions. In the first reaction (EC 6.3.2.31, coenzymeF420-0-L-glutamate ligase) the enzyme attaches a glutamate via its α-amine group to F420-0. In the second reaction, which is described here, the enzyme catalyses the addition of a second L-glutamate residue to the γ-carboxyl of the first glutamate. Group: Enzymes. Synonyms: F420:γ-glutamyl ligase; CofE-AF; MJ0768; CofE. Enzyme Commission Number: EC 6.3.2.34. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5751; coenzymeF420-1:γ-L-glutamate ligase; EC 6.3.2.34; F420:γ-glutamyl ligase; CofE-AF; MJ0768; CofE. Cat No: EXWM-5751.
coenzymeF420H2 oxidase
The enzyme contains FMN and a binuclear iron center. The enzyme from the archaeon Methanothermobacter marburgensis is Si-face specific with respect to C-5 of coenzymeF420. Group: Enzymes. Synonyms: FprA. Enzyme Commission Number: EC 1.5.3.22. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-1550; coenzymeF420H2 oxidase; EC 1.5.3.22; FprA. Cat No: EXWM-1550.
coenzymeF420 hydrogenase
An iron-sulfur flavoprotein (FAD) containing nickel. The enzyme from some sources contains selenocysteine. The enzyme also reduces the riboflavin analogue of F420, flavins and methylviologen, but to a lesser extent. The hydrogen acceptor coenzymeF420 is a deazaflavin derivative. Group: Enzymes. Synonyms: 8-hydroxy-5-deazaflavin-reducing hydrogenase; F420-reducing hydrogenase; coenzymeF420-dependent hydrogenase. Enzyme Commission Number: EC 1.12.98.1. CAS No. 9027-5-8. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-0524; coenzymeF420 hydrogenase; EC 1.12.98.1; 9027-05-8; 8-hydroxy-5-deazaflavin-reducing hydrogenase; F420-reducing hydrogenase; coenzymeF420-dependent hydrogenase. Cat No: EXWM-0524.
coenzymeF420 oxidoreductase (ferredoxin)
The enzyme from the archaeon Methanosarcina mazei contains iron-sulfur centres and FAD. Group: Enzymes. Synonyms: Fd:F420 oxidoreductase; FpoF protein; ferredoxin:F420 oxidoreductase. Enzyme Commission Number: EC 1.5.7.2. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-1559; coenzymeF420 oxidoreductase (ferredoxin); EC 1.5.7.2; Fd:F420 oxidoreductase; FpoF protein; ferredoxin:F420 oxidoreductase. Cat No: EXWM-1559.
coenzyme γ-F420-2:α-L-glutamate ligase
The enzyme caps the γ-glutamyl tail of the hydride carrier coenzymeF420. Group: Enzymes. Synonyms: MJ1001; CofF protein; γ-F420-2:α-L-glutamate ligase. Enzyme Commission Number: EC 6.3.2.32. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5749; coenzyme γ-F420-2:α-L-glutamate ligase; EC 6.3.2.32; MJ1001; CofF protein; γ-F420-2:α-L-glutamate ligase. Cat No: EXWM-5749.
formate dehydrogenase (coenzymeF420)
The enzyme from methanogenic archaea is a involved in formate-dependent H2 production. It contains noncovalently bound FAD. Group: Enzymes. Synonyms: coenzymeF420 reducing formate dehydrogenase; coenzymeF420-dependent formate dehydrogenase. Enzyme Commission Number: EC 1.2.99.9. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-1237; formate dehydrogenase (coenzymeF420); EC 1.2.99.9; coenzymeF420 reducing formate dehydrogenase; coenzymeF420-dependent formate dehydrogenase. Cat No: EXWM-1237.
glucose-6-phosphate dehydrogenase (coenzyme-F420)
The enzyme is very specific for D-glucose 6-phosphate. No activity with NAD+, NADP+, FAD and FMN. Group: Enzymes. Synonyms: coenzymeF420-dependent glucose-6-phosphate dehydrogenase; F420-dependent glucose-6-phosphate dehydrogenase; FGD1; Rv0407; F420-dependent glucose-6-phosphate dehydrogenase 1. Enzyme Commission Number: EC 1.1.98.2. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-0439; glucose-6-phosphate dehydrogenase (coenzyme-F420); EC 1.1.98.2; coenzymeF420-dependent glucose-6-phosphate dehydrogenase; F420-dependent glucose-6-phosphate dehydrogenase; FGD1; Rv0407; F420-dependent glucose-6-phosphate dehydrogenase 1. Cat No: EXWM-0439.
secondary-alcohol dehydrogenase (coenzyme-F420)
The enzyme isolated from the methanogenic archaea Methanogenium liminatans catalyses the reversible oxidation of various secondary and cyclic alcohols to the corresponding ketones. Group: Enzymes. Synonyms: F420-dependent alcohol dehydrogenase; secondary alcohol:F420 oxidoreductase; F420-dependent secondary alcohol dehydrogenase. Enzyme Commission Number: EC 1.1.98.5. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-0442; secondary-alcohol dehydrogenase (coenzyme-F420); EC 1.1.98.5; F420-dependent alcohol dehydrogenase; secondary alcohol:F420 oxidoreductase; F420-dependent secondary alcohol dehydrogenase. Cat No: EXWM-0442.
sulfite reductase (coenzymeF420)
The enzyme, isolated from the archaeon Methanocaldococcus jannaschii, is involved in sulfite detoxification and assimilation. Group: Enzymes. Synonyms: coenzymeF420-dependent sulfite reductase; Fsr. Enzyme Commission Number: EC 1.8.98.3. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-1684; sulfite reductase (coenzymeF420); EC 1.8.98.3; coenzymeF420-dependent sulfite reductase; Fsr. Cat No: EXWM-1684.
2-phospho-L-lactate guanylyltransferase
This enzyme is involved in the biosynthesis of coenzymeF420, a redox-active cofactor found in all methanogenic archaea, as well as some eubacteria. Group: Enzymes. Synonyms: CofC; MJ0887. Enzyme Commission Number: EC 2.7.7.68. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-3279; 2-phospho-L-lactate guanylyltransferase; EC 2.7.7.68; CofC; MJ0887. Cat No: EXWM-3279.
2-phospho-L-lactate transferase
This enzyme is involved in the biosynthesis of coenzymeF420, a redox-active cofactor found in all methanogenic archaea, as well as some eubacteria. Group: Enzymes. Synonyms: LPPG:Fo 2-phospho-L-lactate transferase; LPPG:7,8-didemethyl-8-hydroxy-5-deazariboflavin 2-phospho-L-lactate transferase; MJ1256; lactyl-2-diphospho-(5')guanosine:Fo 2-phospho-L-lactate transferase; CofD. Enzyme Commission Number: EC 2.7.8.28. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-3327; 2-phospho-L-lactate transferase; EC 2.7.8.28; LPPG:Fo 2-phospho-L-lactate transferase; LPPG:7,8-didemethyl-8-hydroxy-5-deazariboflavin 2-phospho-L-lactate transferase; MJ1256; lactyl-2-diphospho-(5')guanosine:Fo 2-phospho-L-lactate transferase; CofD. Cat No: EXWM-3327.
5,10-methylenetetrahydromethanopterin reductase
Catalyses an intermediate step in methanogenesis from CO2 and H2 in methanogenic archaea. Group: Enzymes. Synonyms: 5,10-methylenetetrahydromethanopterin cyclohydrolase; N5,N10-methylenetetrahydromethanopterin reductase; methylene-H4MPT reductase; coenzymeF420-dependent N5,N10-methenyltetrahydromethanopterin reductase; N5, N10-methylenetetrahydromethanopterin: coenzyme-F420 oxidoreductase. Enzyme Commission Number: EC 1.5.98.2. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-1565; 5,10-methylenetetrahydromethanopterin reductase; EC 1.5.98.2; 5,10-methylenetetrahydromethanopterin cyclohydrolase; N5,N10-methylenetetrahydromethanopterin reductase; methylene-H4MPT reductase; coenzymeF420-dependent N5,N10-methenyltetrahydromethanopterin reductase; N5, N10-methylenetetrahydromethanopterin: coenzyme-F420 oxidoreductase. Cat No: EXWM-1565.
Binds a [4Fe-4S] cluster. The cluster is coordinated by 3 cysteines and an exchangeable AdoMet molecule. The first stage of catalysis is reduction of the 2 AdoMet to produce 2 methionine and 2 5'-deoxyadenosin-5-yl radicals that extract a hydrogen from each of the substrates permitting the condensation of the two. The overall reaction catalysed is the transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino-6-ribitylaminopyrimidine-2,4(1H,3H)-dione to form 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO). 7,8-Didemethyl-8-hydroxy-5-deazariboflavin is the chromophore of the hydride carrier coenzymeF420. Group: Enzymes. Synonyms: FO synthase. Enzyme Commission Number: EC 2.5.1.77. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-2815; 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase; EC 2.5.1.77; FO synthase. Cat No: EXWM-2815.
F420H2:quinone oxidoreductase
An enzyme complex that contains FAD and iron-sulfur clusters. The enzyme has been described in the archaea Methanosarcina mazei and Archaeoglobus fulgidus. Group: Enzymes. Synonyms: FqoF protein. Enzyme Commission Number: EC 1.1.98.4. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-0441; F420H2:quinone oxidoreductase; EC 1.1.98.4; FqoF protein. Cat No: EXWM-0441.
fumarate reductase (CoM/CoB)
The enzyme, isolated from the archaeon Methanobacterium thermoautotrophicum, is very oxygen sensitive. It cannot use reduced flavins, reduced coenzymeF420, or NAD(P)H as an electron donor. Distinct from EC 1.3.1.6 [fumarate reductase (NADH)], EC 1.3.5.1 [succinate dehydrogenase (ubiquinone)], and EC 1.3.5.4 [fumarate reductase (quinol)]. Group: Enzymes. Synonyms: thiol:fumarate reductase; Tfr. Enzyme Commission Number: EC 1.3.4.1. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-1384; fumarate reductase (CoM/CoB); EC 1.3.4.1; thiol:fumarate reductase; Tfr. Cat No: EXWM-1384.
methylenetetrahydromethanopterin dehydrogenase
CoenzymeF420 is a 7,8-didemethyl-8-hydroxy-5-deazariboflavin derivative; methanopterin is a pterin analogue. The enzyme is involved in the formation of methane from CO2 in the methanogen Methanothermobacter thermautotrophicus. Group: Enzymes. Synonyms: N5,N10-methylenetetrahydromethanopterin dehydrogenase; 5,10-methylenetetrahydromethanopterin dehydrogenase. Enzyme Commission Number: EC 1.5.98.1. CAS No. 100357-01-5. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-1564; methylenetetrahydromethanopterin dehydrogenase; EC 1.5.98.1; 100357-01-5; N5,N10-methylenetetrahydromethanopterin dehydrogenase; 5,10-methylenetetrahydromethanopterin dehydrogenase. Cat No: EXWM-1564.
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