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X-Gal (5-Bromo-4-chloro-3-indolyl-b-D-galactoside galactopyranoside, Xgal) Quick inquiry Where to buy Suppliers range | Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white.Gene Cloning:In gene cloning, X-gal is used as a visual indication of whether a cell expresses a functional β-galactosidase enzyme in a technique called blue/white screening. This method of screening is a convenient way of distinguishing a successful cloning product from other unsuccessful ones.The blue/white screening method relies on the principle of α-complementation of the β-galactosidase gene, where a fragment of the lacZ gene (lacZα) in the plasmid can complement another mutant lacZ gene (lacZΔM15) in the cell. Both genes by themselves produce non-functional peptides, however, when expressed together, as when a plasmid containing lacZα is transformed into a lacZΔM15 cells, they form a functional β-galactosidase. The presence of an active β-galactosidase may be detected when cells are grown in plates containing X-gal, the blue-colored product precipitated within cells resulted in the characteristic blue colonies. However, the multiple cloning site, where a gene of interest may be ligated into the plasmid vector, is located within the lacZα gene.... . Group: Biochemicals. Alternative Names: X-Gal, 5-Bromo-4-chloro-3-indolyl-b-D-galactoside (galactopyranoside), BCIG. Grades: Molecular Biology Grade. CAS No. 7240-90-6. Pack Sizes: 100mg, 250mg, 1g, 10g. Molecular Formula: C14H15BrClNO6, Molecular Weight: 408.61. US Biological Life Sciences. | Worldwide |