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Ficin. Group: Molecular Biology. CAS No. 9001-33-6. Pack Sizes: 25g. US Biological Life Sciences.
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Ficin froM Fig Tree Latex
Ficin froM Fig Tree Latex. Synonyms: E.C. 3.4.4.12;FIG TREE LATEX;FICIN;EC 3.4.22.3;EC: 3.4.22.3;DIGEST-ALL(TM) 1;FICIN,POWDER;Ficin Agarose from fig tree latex. CAS No. 9001-33-6. Pack Sizes: 1 g. Product ID: CDF4-0051. Category: Enzyme Preparations. Product Keywords: Food Ingredients; Enzyme Preparations; Ficin froM Fig Tree Latex; CDF4-0051; 9001-33-6; 9001-33-6; 9001-33-6. Purity: 0.99. Color: tan. EC Number: 9001-33-6. Physical State: Suspension. Storage: -20°C.
Immobilized Ficin (Agarose Resin)
Immobilized Ficin specifically cleaves mouse IgG1 into F(ab?)2 or Fab fragments in the presence of 4mM or 25mM cysteine, respectively. Fragment generation from other species and isotypes may also be possible through modification of the cysteine concentration and other digestion parameters. Ficin, isolated from fig latex, is a sulfhydryl protease with a similar active site to bromelain and papain; however, yields and immunoreactivity are better when digesting mouse IgG1 with ficin. Ficin acts on bonds involving uncharged and aromatic amino acids. Ficin (25K) is effective at pH 4-9.5 with an optimum pH of 6.5. Ficin immobilization enhances stability against denaturation, heat and autolysis. Immobilization also eliminates the potential for antibody-enzyme adducts that result in continued sample digestion. Additionally, immobilization allows ficin to be reused. Group: Enzymes. Synonyms: Immobilized Ficin. Enzyme Commission Number: EC 3.4.22.3. Ficin. Activity: ~1.2mg/mL of settled resin. Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C. Form: 33% slurry (i.e., 5mL of settled resin is equal to 15mL of slurry). Immobilized Ficin (Agarose Resin); Protein Fragmentation Enzymes Kits; antibody fragmentation; Immobilized Ficin; Ficin. Cat No: NATE-1865.
Native Fig tree latex Ficin
Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab')2 fragments from mouse IgG1. Group: Enzymes. Synonyms: ficin; debricin; higueroxyl delabarre; EC 3.4.22.3; 9001-33-6; ficain. Enzyme Commission Number: EC 3.4.22.3. CAS No. 9001-33-6. Ficin. Mole weight: 23.8 kDa. Activity: > 1.0 units/mg protein. Form: saline suspension or lyophilized powder. Source: Fig tree latex. ficin; debricin; higueroxyl delabarre; EC 3.4.22.3; 9001-33-6; ficain. Cat No: NATE-0255.
ficain
The major proteolytic component of the latex of fig, Ficus glabrata. Cysteine endopeptidases with similar properties are present in other members of the large genus Ficus. In peptidase family C1 (papain family). Group: Enzymes. Synonyms: ficin; debricin; higueroxyl delabarre. Enzyme Commission Number: EC 3.4.22.3. CAS No. 9001-33-6. Ficin. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-4204; ficain; EC 3.4.22.3; 9001-33-6; ficin; debricin; higueroxyl delabarre. Cat No: EXWM-4204.
Ficain
Ficain is an enzyme extract composed of several proteases that can be isolated from Ficus hispida L. and the latex of fig ( Ficus carica ). Ficain has different specificities in different proportions during fruit ripening. Ficain is widely used in protein hydrolysis, food, production of bioactive peptides and antibody fragments [1] [2]. Uses: Scientific research. Group: Signaling pathways. Alternative Names: Ficin. CAS No. 9001-33-6. Pack Sizes: 1 g. Product ID: HY-P3028.
Tosyl phenylalanyl chloromethyl ketone
Tosyl phenylalanyl chloromethyl ketone (TPCK) is an irreversible inhibitor of chymotrypsin-like proteases. It also inhibits some cysteine proteases such as caspase, papain, bromelain or ficin. Uses: Alkylating agents. Synonyms: Nα-Tosyl-L-Phenylalanine Chloromethyl Ketone; TPCK; Tos-Phe-CH2Cl. Grades: ≥98%. CAS No. 402-71-1. Molecular formula: C17H18ClNO3S. Mole weight: 351.8.
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