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Immobilized Trypsin, TPCK Treated (Agarose Resin) Trypsin immobilized on beaded agarose makes it possible to eliminate enzyme contamination of tryptic digests. The trypsin can be easily removed from the digest by separating the trypsin gel from the digestion solution. The Thermo Scientific Immobilized TPCK Trypsin is treated with L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK), which is a reagent that has been reported to inhibit chymotrypsin activity without effect on trypsin. Trypsin is a 23,200 molecular weight protein with a pH optimum between 7.5 and 9.0. The isoelectric points of trypsinogen and trypsin are 10.5 and 9.3, respectively. Trypsin has a wide range of applications including amino acid analys...estimated from the number of lysine and arginine residues in the protein. Ionexchange chromatography, paper electrophoresis or peptide mapping can be used to separate digestion fragments. Group: Enzymes. Synonyms: Immobilized Trypsin. Enzyme Commission Number: EC 3.4.21.4. Trypsin. Activity: > 200 TAME units per mL of gel. Storage: Upon receipt store at 4°C. Product is shipped at ambient temperature. Form: 2mL of settled gel supplied as a 50% slurry containing glycerol and 0.05% sodium azide as a preservative. Source: Bovine pancreas. Immobilized Trypsin, TPCK Treated (Agarose Resin); Immobilized Trypsin; Trypsin; Protein Fragmentation Enzymes Kits. Cat No: NATE-1867. Creative Enzymes
Immobilized bovine trypsin Immobilized bovine trypsin is ideal for digestions of proteins and peptides. Trypsin is a protease that cleaves peptide bonds at Arg and Lys. Trypsin protease is TPCK treated to remove chymotryptic activity before immobilization. Trypsin can be used to provide complementary sequence coverage when compared to chymotryptic digested samples. Group: Enzymes. Synonyms: α-trypsin; β-trypsin; cocoonase; parenzyme; parenzymol; tryptar; trypure; pseudotrypsin; tryptase; tripcellim; sperm receptor hydrolase; Alpha-trypsin; Beta-trypsin; EC 3. 4. 21. 4; Trypsin. Enzyme Commission Number: EC 3. 4. 21. 4. Purity: >95% by SDS-PAGE analysis. Mole weight: 23300. Stability: 12 months from delivery. Storage: 4°C. Form: Resin. Source: Bovine pancreas. Species: Bovine. α-trypsin; β-trypsin; cocoonase; parenzyme; parenzymol; tryptar; trypure; pseudotrypsin; tryptase; tripcellim; sperm receptor hydrolase; Alpha-trypsin; Beta-trypsin; EC 3. 4. 21. 4; Trypsin; Immobilized trypsin. Cat No: NATE-1755. Creative Enzymes
Immobilized TPCK-Trypsin on F7m Trypsin hydrolyzes proteins, peptides, amides and esters specifically at the carboxyl groups of the basic amino acids L-arginine or L-lysine. F7m: 1. 0 mg trypsin per CR-column, immobilized on polyvinyl10,200 ST-units immobilized per CR-column. Nr. 15 Storage buffer: 50 mM Tris/HCl at pH 8. 0 at 4°CNr. 67 Reaction buffer: 50 mM phosphate at pH 8. 0Nr. 68 Washing buffer: 50 mM phosphate at pH 8. 0, 1 M NaCl. Group: Enzymes. Synonyms: α-trypsin; β-trypsin; cocoonase; parenzyme; parenzymol; tryptar; trypure; pseudotrypsin; tryptase; tripcellim; sperm receptor hydrolase; Alpha-trypsin; Beta-trypsin; EC 3. 4. 21. 4; Trypsin. Enzyme Commission Number: EC 3. 4. 21. 4. Storage: 4 °C. Source: Bovine pancreas. α-trypsin; β-trypsin; cocoonase; parenzyme; parenzymol; tryptar; trypure; pseudotrypsin; tryptase; tripcellim; sperm receptor hydrolase; Alpha-trypsin; Beta-trypsin; EC 3. 4. 21. 4; Trypsin; Immobilized TPCK-Trypsin; Immobilized Trypsin; TPCK-Trypsin. Cat No: NATE-1771. Creative Enzymes
Immobilized TPCK-Trypsin on G3m Trypsin hydrolyzes proteins, peptides, amides and esters specifically at the carboxyl groups of the basic amino acids L-arginine or L-lysine. G3m: 25 ug trypsin per CR-column, immobilized on dextran. 260 ST-units immobilized per CR-column. This CR-column cuts at least 50 ug tubulin per application. Nr. 15 Storage buffer: 50 mM Tris/HCl at pH 8. 0Nr. 67 Reaction buffer: 50 mM phosphate at pH 8. 0 (S?rensen)Nr. 68 Washing buffer: 50 mM phosphate at pH 8. 0, 1 M NaCl. Group: Enzymes. Synonyms: α-trypsin; β-trypsin; cocoonase; parenzyme; parenzymol; tryptar; trypure; pseudotrypsin; tryptase; tripcellim; sperm receptor hydrolase; Alpha-trypsin; Beta-trypsin; EC 3. 4. 21. 4; Trypsin. Enzyme Commission Number: EC 3. 4. 21. 4. Storage: 4 °C. Source: Bovine pancreas. α-trypsin; β-trypsin; cocoonase; parenzyme; parenzymol; tryptar; trypure; pseudotrypsin; tryptase; tripcellim; sperm receptor hydrolase; Alpha-trypsin; Beta-trypsin; EC 3. 4. 21. 4; Trypsin; Immobilized TPCK-Trypsin; Immobilized Trypsin; TPCK-Trypsin. Cat No: NATE-1772. Creative Enzymes

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