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| Product | Description | |
|---|---|---|
| lacto-N-biosidase | The enzyme from Streptomyces specifically hydrolyses the terminal lacto-N-biosyl residue (β-D-Gal-(1?3)-D-GlcNAc) from the non-reducing end of oligosaccharides with the structure β-D-Gal-(1?3)-β-D-GlcNAc-(1?3)-β-D-Gal-(1?R). Lacto-N-hexaose (β-D-Gal-(1?3)-β-D-GlcNAc-(1?3)-β-D-Gal-(1?3)-β-D-GlcNAc-(1?3)-β-D-Gal-(1?4)-D-Glc) is hydrolysed to form first lacto-N-tetraose plus lacto-N-biose, with the subsequent formation of lactose. Oligosaccharides in which the non-reducing terminal Gal or the penultimate GlcNAc are replaced by fucose or sialic acid are not substrates. Asialo GM1 tetraose (β-D-Gal-(1?3)-β-D-GalNAc-(1?3)-β-D-Gal-(1?4)-D-Glc) is hydrolysed very slowly, but lacto-N-neotetraose (β-D-Gal-(1?4)-β-D-GalNAc-(1?3)-β-D-Gal-(1?4)-D-Glc) is not a substrate. Group: Enzymes. Enzyme Commission Number: EC 3.2.1.140. CAS No. 146359-52-6. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-3823; lacto-N-biosidase; EC 3.2.1.140; 146359-52-6. Cat No: EXWM-3823. |  |
| Lacto-N-Biosidase, Recombinant | Lacto-N-Biosidase may be used during glycoprotein analysis to better study the structure and function of glycoprotein and glycolipid sugar chains. This product can be used for specific oligosaccharide hydrolysis of type-I chain oligosaccharides, producing lacto-N-biose (Gal β 1-3 GlcNAc) as a byproduct, but not oligosaccharide hydrolysis of type-II chain oligosaccharides. As a result, Lacto-N-Biosidase can be used to distinguish type-I versus type-II glycoprotein as well as glycolipid sugar chains. When used in conjunction with α -1,3/4-Fucosidase, Lacto-N-Biosidase can also help distinguish Sialyl-Lewis x and Sialyl-Lewis a structures. Applications: Specific hydrolysis of oligosaccharides with type-i sugar chains and production of byproduct lacto-n-biose (gal β 1-3 glcnac). Group: Enzymes. Synonyms: Oligosaccharide lacto-N-biosylhydrolase; Lacto-N-Biosidase. Lacto-N-Biosidase. Mole weight: 60 kDa (SDS-PAGE). Form: Solution in 50 mM sodium acetate buffer, pH 5.5, containing 0.05% Brij-58. Source: Streptomyces sp. 142. Oligosaccharide lacto-N-biosylhydrolase; Lacto-N-Biosidase. Cat No: NATE-0855. | |
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