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| Product | Description | |
|---|---|---|
| T4 DNA Ligase | T4 DNA Ligase. T4 dna ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex dna or rna with blunt or cohesive end. the enzyme repairs single-strand nicks in duplex dna, rna or dna/rna hybrids but has no activity on single-strand nucleic acids. t4 dna ligase requires atp as a cofactor. Group: DNA Modifying Enzymes. Purity: 10KU; 20KU. Storage: Store at -20°C. Cat No: ME-4005. |  |
| DNA Ligase T4 Reaction Buffer, 10X | DNA Ligase T4 Reaction Buffer, 10X. Group: Molecular Biology. Grades: Molecular Biology Grade. CAS No. 9015-85-4. Pack Sizes: 1.5ml. US Biological Life Sciences. |  Worldwide |
| DNA Ligase, T4, Recombinant, BioAssay Kit, 10mM ATP | DNA Ligase, T4, Recombinant, BioAssay Kit, 10mM ATP. Group: Molecular Biology. Grades: Purified. Pack Sizes: 100ul. US Biological Life Sciences. | Worldwide |
| DNA Ligase, T4, Recombinant, BioAssay Kit, Reaction Buffer (10X) | DNA Ligase, T4, Recombinant, BioAssay Kit, Reaction Buffer (10X). Group: Molecular Biology. Grades: Purified. Pack Sizes: 1ml. US Biological Life Sciences. | Worldwide |
| DNA Ligase, T4, Recombinant, BioAssay Kit, T4 DNA Ligase | DNA Ligase, T4, Recombinant, BioAssay Kit, T4 DNA Ligase. Group: Molecular Biology. Grades: Purified. Pack Sizes: 20KU. US Biological Life Sciences. | Worldwide |
| Abs I | One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19SE/DriI -digest in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 2-fold overdigestion with enzyme about 90% of the dna fragments can be ligated with t4 dna ligase at 16°c and recut. Group: Restriction Enzymes. Purity: 50U; 250U. CC↑TCGAGG GGAGCT↓CC. Activity: 500-1000u.a./ml. Appearance: 10 X SE-buffer AbsI. Storage: -20°C. Form: Liquid. Source: Arthrobacter species 7M06. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Cat No: RE-1002EN. | |
| AclW I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 3-fold overdigestion with enzyme about 50% of the dna fragments can be ligated with t4 dna ligase and recut. in the presence of 10% peg ligation is better. Group: Restriction Enzymes. Purity: 100U; 500U. GGATC(N)4↑ CCTAG(N)5&darr. Activity: 1000-3000u.a./ml. Appearance: 10 X SE-buffer Y, BSA. Storage: -20°C. Form: Liquid. Source: Acinetobacter calcoaceticus W2131. Pack: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Cat No: RE-1010EN. | |
| Aco I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA(dam-, dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 3-fold overdigestion with acoi more than 90% of lambda dna fragments can be ligated with t4 dna ligase at 16°c and recut. Group: Restriction Enzymes. Y↑GGCCR RCCGG↓Y. Appearance: 10 X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: Acinetobacter calcoaceticus. Pack: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Cat No: RE-1011EN. | |
| AluB I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 10-fold overdigestion with enzyme 80% of the lambda dna fragments can be ligated with t4 dna ligase and can be recut. Group: Restriction Enzymes. Purity: 200U; 1000U. AG↑CT TC↓GA. Activity: 5000u.a./ml. Appearance: 10 X SE-buffer B, BSA. Storage: -20°C. Form: Liquid. Source: Arthrobacter luteus B. Pack: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Cat No: RE-1019EN. | |
| Bse21 I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 2-fold overdigestion with enzyme about 50% of dna fragments can be ligated by using of high concentration t4 dna ligase with presence of 10% peg. of these more than 90% can be recut. Group: Restriction Enzymes. Purity: 500 U; 2500U. CC↑TNAGG GGANT↓CC. Activity: 20000u.a./ml. Appearance: 10 X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: Bacillus species 21. Pack: 10 mM KH2PO4 (pH 7.4); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1045RE. | |
| BspAC I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 5-fold overdigestion with enzyme more than 95% of the lambda dna fragments can be ligated with t4 dna ligase at 16°c and 50% of these can be recut. Group: Restriction Enzymes. Purity: 200U; 1000U. C↑CGC GGC↓G. Activity: 2000-5000u.a./ml. Appearance: 10 X SE-buffer O, BSA. Storage: -20°C. Form: Liquid. Source: Bacillus species AC. Pack: 10 mM KH2PO4(pH 7.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1055RE. | |
| Bsu I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 5-fold overdigestion with enzyme ~10% of dna fragments can be ligated with t4 dna ligase and recut. Group: Restriction Enzymes. Purity: 200U; 1000U. GTATCC(N)6↑ CATAGG(N)5&darr. Activity: 2000-5000u.a./ml. Appearance: 10 X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: Bacillus sphaericus. Pack: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 0.05% Triton X-100, 50% glycerol. Cat No: ET-1091RE. | |
| FaeI | One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 3-fold overdigestion with enzyme more then 90% of the dna fragments can be ligated with t4 dna ligase at 16°c and recut. Group: Restriction Enzymes. Purity: 500 U; 2500U. CATG↑ ↓GTAC. Activity: 500-2000 u.a./ml. Appearance: 10 X SE-buffer FaeI, BSA. Storage: -20°C. Form: Liquid. Source: Flavobacterium aquatile N3. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1105RE. | |
| HpySE526 I | One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 5-fold overdigestion with enzyme about 95% of the dna fragments can be ligated with t4 dna ligase and recut. Group: Restriction Enzymes. Purity: 200U; 1000U. A↑CGT TGC↓A. Activity: 5000u.a./ml. Appearance: 10 X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: An E.coli strain, that carries the cloned HpySE 526I gene from Helicobacter pylori SE526. Pack: 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol. Cat No: ET-1123RE. | |
| Mfe I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 20-fold overdigestion with enzyme 90% of the dna fragments can be ligated with t4 dna ligase and recut. Group: Restriction Enzymes. Purity: 1000U; 5000U. C↑AATTG GTTAA↓C. Activity: 20000u.a./ml. Appearance: 10 X SE-buffer B, BSA. Storage: -20°C. Form: Liquid. Source: An E.coli strain, that carries the cloned gene Mfe I from Mycoplasma fermentans. Pack: 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1131RE. | |
| PciS I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 3-fold overdigestion with enzyme 90% of the dna fragments can be ligated with t4 dna ligase at 16°c and 95% of these can be recut. Group: Restriction Enzymes. Purity: 50U; 250U. GCTCTTCN↑ CGAGAAG(N)4&darr. Activity: 500-2000 u.a./ml. Appearance: 10 X SE-buffer B. Storage: -20°C. Form: Liquid. Source: Planococcus citreus S. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA and 50% glycerol. Cat No: ET-1146RE. | |
| PstN I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 5-fold overdigestion with enzyme > 95% of lambda dna fragments can be ligated with t4 dna ligase and recut. Group: Restriction Enzymes. Purity: 500 U; 2500U. CAGNNN↑CTG GTC↓NNNGAC. Activity: 5000-10000u.a./ml. Appearance: 10 X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: Pseudomonas stutzeri 217. Pack: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Cat No: ET-1162RE. | |
| Rig I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Ad2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 3-fold overdigestion with enzyme > 95% of ad2 dna fragments can be ligated with t4 dna ligase and recut. Group: Restriction Enzymes. Purity: 100U; 500U. GGCCGG↑CC CC↓GGCCGG. Activity: 2000u.a./ml. Appearance: 10 X SE-buffer RigI, BSA. Storage: -20°C. Storage at -70°C is recommended for periods longer than 7 days. Form: Liquid. Source: Rhizobium yangligense. Pack: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1165RE. | |
| RsaN I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 5-fold overdigestion with enzyme more than 90% of the dna fragments can be ligated with t4 dna ligase and recut. Group: Restriction Enzymes. Purity: 200U; 1000U. G↑TAC CAT↓G. Activity: 5000u.a./ml. Appearance: 10 X SE-buffer B. Storage: -20°C. Form: Liquid. Source: Rhodopseudomonas sphaeroides N. Pack: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol. Cat No: ET-1167RE. | |
| SCR7 Pyrazine | SCR7 pyrazine also is a potent inhibitor of nonhomologous endjoining (NHEJ) mediated by DNA ligase IV. It enhances CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency in vitro upt to 19-fold. SCR7 pyrazine is a product of spontaneous cyclization of CRISPR enhancer SCR7 first reported by Srivastava, M., et al.DNA ligase IV seals double-strand breaks during the process of nonhomologous end-joining in DNA repair. Inhibiting this function in cancer cells is one strategy to prevent deleterious cell growth. SCR7 is a small molecule inhibitor of DNA ligase IV that prevents nonhomologous end-joining by interfering with ligase binding and activating apoptosis.1 It also inhibits ligase III, but does not affect the activity of T4 DNA ligase or ligase I. SCR7 has been used to increase the rate of homology directed repair triggered by DNA double-strand breaks and to inhibit cancer cell growth in vitro (IC50s = 8-120 µM) and in mouse models when co-administered with double-strand break-inducing therapeutic compounds.1,2,3,4. Group: Biochemicals. Alternative Names: 2,3-Dihydro-6,7-diphenyl-2-thioxo-4 (1H)-pteridinone; 6,7-Diphenyl-2-thio-lumazine. Grades: Highly Purified. CAS No. 14892-97-8. Pack Sizes: 10mg. Molecular Formula: C??H??N?OS, Molecular Weight: 332.38. US Biological Life Sciences. | Worldwide |
| Set I | One unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the below indicated structure in 1 hour at 55°C in a total reaction volume of 20 μl. Note! seti cleaves a canonical site and several other sites with a weaker activity. in the case of long incubation with seti dna can be digested to small oligos. Applications: After 5-fold overdigestion with enzyme, approximately 50% of the pbr322 dna fragments can be ligated with t4 dna ligase and recut. Group: Restriction Enzymes. Purity: 200U; 1000U. ASST↑ ↓TSSA. Activity: 5000u.a./ml. Appearance: 10 X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned Set I gene from Streptomyces werraensis 37. Pack: 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol. Cat No: ET-1171RE. | |
| Sma I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 25°C in a total reaction volume of 50 μl. Applications: After 20-fold overdigestion with enzyme more than 90% of the dna fragments can be ligated (by using of high concentration t4 dna ligase and 10% peg) and recut. Group: Restriction Enzymes. Purity: 2000U; 10000U. CCC↑GGG GGG↓CCC. Activity: 20000u.a./ml. Appearance: 10 X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned Sma I gene from Serratia marcescens. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1176RE. | |
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