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T7 RNA polymerase T7 RNA polymerase is a polymerase expressed by Escherichia coli from the RNA polymerase gene of T7 bacteriophage. T7 RNA polymerase is highly specific and involved in in vitro transcription (IVT) of mRNA. In the presence of Mg 2+ , T7 RNA polymerase only uses the single-stranded or double-stranded DNA containing the T7 promoter sequence as a template, and uses NTP as a substrate to synthesize RNA complementary to the single-stranded DNA downstream of the promoter [1] [2]. Uses: Scientific research. Group: Signaling pathways. CAS No. 9014-24-8. Pack Sizes: 1 KU; 5 KU. Product ID: HY-E70090. MedChemExpress MCE
Modified T7 RNA polymerase A modified T7 RNA polymerase that orthogonally recognizes a non-natural promoter sequence: 5-TAATACCGGTCACTATA, where the underlined residues differ from wild-type. Group: Enzymes. Synonyms: CGG-R12-KIR. Enzyme Commission Number: EC 2.7.7.6. CAS No. 9014-24-8. Purity: > 98% (SDS-PAGE). Mole weight: 100.142 kDa. Storage: at -20 °C. Form: Liquid. CGG-R12-KIR; Non-Natural Promoter T7 Polymerase; modified T7 RNA polymerase; T7 RNA polymerase. Cat No: NATE-1698. Creative Enzymes
5-Azido-C3-UTP 5-Azido-C3-UTP can be incorporated into RNA by T7 RNA polymerase-mediated in vitro transcription. Synonyms: 5-(3-Azidopropyl)-uridine-5'-triphosphate, Triethylammonium salt. Grades: ≥ 95% by HPLC. Molecular formula: C12H20N5O15P3 (free acid). Mole weight: 567.23 (free acid). BOC Sciences
5-Methoxyuridine 5'-triphosphate trisodium 5-Methoxyuridine-5'-Triphosphate trisodium is a modified nucleotide triphosphate (NTP). 5-Methoxyuridine-5'-Triphosphate trisodium can be added into mRNA with T7 RNA polymerase. Synonyms: 5-Methoxy-UTP trisodium; 5-OMe-UTP trisodium. Molecular formula: C10H14N2Na3O16P3. Mole weight: 580.11. BOC Sciences
5-Propargylamino-CTP - ATTO-488 5-Propargylamino-CTP - ATTO-488 is a biochemical reagent used for labeling RNA transcripts in vitro. It is incorporated into RNA during transcription by T7 RNA polymerase and can be used for in vitro enzymatic synthesis of labeled RNA molecules for various downstream applications, including RNA aptamer selection, RNA imaging, and RNA-protein interaction studies. Synonyms: 5-Propargylamino-cytidine-5'-triphosphate, labeled with ATTO 488, Triethylammonium salt. Grades: ≥ 95% by HPLC. Molecular formula: C37H40N7O23P3S3 (free acid). Mole weight: 1107.08 (free acid). BOC Sciences 2
Aminoallyl-UTP - 6-FAM Aminoallyl-UTP - 6-FAM serves as a game-changing tool in molecular biology for the labeling of RNA with fluorescent dyes. Incorporating itself seamlessly into RNA by T7, SP6 or T3 RNA polymerases, it has expansive usage, catering to a niche demand of detecting RNA expression levels in vitro. Gene expression studies, RNA interference research, and drug discovery for assorted diseases including cancers, neurodegenerative disorders, and infectious diseases are areas where Aminoallyl-UTP - 6-FAM shows true prowess. Synonyms: 5-(3-Aminoallyl)-uridine-5'-triphosphate, labeled with 6 FAM, Triethylammonium salt. Grades: ≥ 95% by HPLC. Molecular formula: C33H30N3O21P3 (free acid). Mole weight: 897.52 (free acid). BOC Sciences
Biotin-14-CTP Biotin-14-CTP is a CTP analog that contains biotin attached at the N4-position via a 14-atom linker. The amount of material provided is sufficient for up to 20 transcription reactions (1ug template) using T7 RNA polymerase. Group: Biochemicals. Grades: Molecular Biology Grade. Pack Sizes: 500nmol. US Biological Life Sciences. USBiological 1
Worldwide
N1-Ethylpseudouridine-5'-Triphosphate A modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-ethylpseudouridine can reduce the immunogenicity of the resulting mRNA. Synonyms: N1-Ethylpseudo-UTP; 1-Ethylpseudo-UTP. Grades: ≥95%. Molecular formula: C11H19N2O15P3. Mole weight: 512.19. BOC Sciences 2
N1-EthylpseudoUridine-5'-Triphosphate Lithium A modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-ethylpseudouridine can reduce the immunogenicity of the resulting mRNA. Synonyms: N1-Ethylpseudo-UTP tetralithium salt; 1-Ethylpseudo-UTP tetralithium salt. Grades: ≥90% by AX-HPLC. Molecular formula: C11H15Li4N2O15P3. Mole weight: 535.92. BOC Sciences 2
N1-Methyl-2'-O-MethylpseudoUridine-5'-Triphosphate Lithium A modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-methylpseudouridine can reduce the immunogenicity of the resulting mRNA. Synonyms: N1,2'-O-Dimethylpseudo-UTP tetralithium salt; N1-Methyl-2'-O-Methylpseudo-UTP tetralithium salt. Grades: ≥90% by AX-HPLC. Molecular formula: C11H15Li4N2O15P3. Mole weight: 535.92. BOC Sciences 3
N1-MethylpseudoUridine-5'-Triphosphate N1-MethylpseudoUridine-5'-Triphosphate is a modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-methylpseudouridine can reduce the immunogenicity of the resulting mRNA. The sodium form N1-MethylpseudoUridine-5'-Triphosphate Sodium (B2706-358101) is in stock. Synonyms: 1-Methylpseudouridine-5'-Triphosphate; N1-Methylpseudo-UTP; 2,4(1H,3H)-Pyrimidinedione, 5-[5-O-[hydroxy[[hydroxy (phosphonooxy) phosphinyl]oxy]phosphinyl]-β -D-ribofuranosyl]-1-methyl-; 1-Methylpseudo-UTP. Grades: ≥95%. CAS No. 1428903-59-6. Molecular formula: C10H17N2O15P3. Mole weight: 498.17. BOC Sciences 2
N1-MethylpseudoUridine-5'-Triphosphate Lithium N1-MethylpseudoUridine-5'-Triphosphate is a modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-methylpseudouridine can reduce the immunogenicity of the resulting mRNA. The sodium form N1-MethylpseudoUridine-5'-Triphosphate Sodium (B2706-358101) is in stock. Synonyms: 1-Methylpseudouridine-5'-Triphosphate lithium salt; N1-Methylpseudo-UTP tetralithium salt; 2,4(1H,3H)-Pyrimidinedione, 5-[5-O-[hydroxy[[hydroxy (phosphonooxy) phosphinyl]oxy]phosphinyl]-β -D-ribofuranosyl]-1-methyl-, lithium salt; 1-Methylpseudo-UTP tetralithium salt. Grades: ≥90% by AX-HPLC. Molecular formula: C10H13Li4N2O15P3. Mole weight: 521.89. BOC Sciences 3
N1-MethylpseudoUridine-5'-Triphosphate Sodium It is a modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-methylpseudouridine can reduce the immunogenicity of the resulting mRNA. The product has passed the functionality test in transcription reaction. The solution is free of DNase and RNase contamination. Synonyms: 1-Methylpseudouridine-5'-Triphosphate sodium salt; N1-Methylpseudo-UTP sodium salt; 2,4(1H,3H)-Pyrimidinedione, 5-[5-O-[hydroxy[[hydroxy (phosphonooxy) phosphinyl]oxy]phosphinyl]-β -D-ribofuranosyl]-1-methyl-, sodium salt; 1-Methylpseudo-UTP sodium salt; 1-Methylpseudouridine-5'-Triphosphate; N1-Methylpseudo-UTP. Grades: 99%, 100mM sodium salt solution. Molecular formula: C10H17N2O15P3.xNa. Mole weight: 498.17 (free acid). BOC Sciences 9
Reverse Transcriptase from HIV, Recombinant Chromatographically purified heterodimer composed of 66kDa and 51kDa subunits. Supplied as a solution in 10mM potassium phosphate, pH 7.4, 1mM DTT and 20% glycerol. Primarily for AIDS research purposes; this enzyme has less fidelity than the AMV enzyme in other applications such as the preparation of cDNA from mRNA for cloning purposes. Applications: Hiv reverse transcriptase is used for research on the aids primer. however it can be substituted for amv reverse transcriptase, which is mainly used to transcribe mrna into double stranded cdna, that can be inserted into prokaryotic vectors. the enzyme can also be used with either single stranded dna or rna templates to mak...or labeling the termini of dna fragments with protruding 5' termini. the enzyme can also be used to sequence dnas by the dideoxy chain termination method of sanger when the klenow fragment of e. coli dna polymerase i, or the t7 dna polymerase yield unsatisfactory results. Group: Enzymes. Synonyms: Reverse transcriptase; RT. Enzyme Commission Number: EC 2.7.7.49. CAS No. 9068-38-6. Purity: Chromatographically purified. RT. Mole weight: 66 kDa and 51 kDa. Activity: > 5,000 units per mg protein. Storage: Store at -20°C. Form: A solution in 10mM potassium phosphate, pH 7.4, 1mM DTT and 20% glycerol. Source: E. coli. Species: HIV. Reverse transcriptase; RT. Cat No: NATE-0987. Creative Enzymes
T7 Tag Peptide T7 Tag Peptide, an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10, and serves as a tag in many expression vectors that is based on the very efficient T7 RNA polymerase expression system. Synonyms: Met-Ala-Ser-Met-Thr-Gly-Gly-Gln-Gln-Met-Gly; L-methionyl-L-alanyl-L-seryl-L-methionyl-(3xi)-L-threonyl-glycyl-glycyl-L-glutaminyl-L-glutaminyl-L-methionyl-glycine. Grades: ≥95%. Molecular formula: C41H71N13O16S3. Mole weight: 1098.27. BOC Sciences 3

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