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| Product | Description | |
|---|---|---|
| tRNA 4-demethylwyosine synthase (AdoMet-dependent) | This enzyme, which is a member of the superfamily of S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes, binds two [4Fe-4S] clusters. Carbons C2 and C3 from pyruvate are incorporated into 4-demethylwyosine. The enzyme is found in eukaryotes where it is part of the pathway for wybutosine synthesis, and in archaea, where it is involved in the biosynthesis of archaeal wye bases, such as wyosine, isowyosine, and methylwyosine. Group: Enzymes. Synonyms: TYW1. Enzyme Commission Number: EC 4.1.3.44. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-4921; tRNA 4-demethylwyosine synthase (AdoMet-dependent); EC 4.1.3.44; TYW1. Cat No: EXWM-4921. |  |
| tRNAPhe (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase | Requires Fe2+. The enzyme is not active with wybutosine. Group: Enzymes. Synonyms: TYW5; tRNA yW-synthesizing enzyme 5. Enzyme Commission Number: EC 1.14.11.42. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-0661; tRNAPhe (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase; EC 1.14.11.42; TYW5; tRNA yW-synthesizing enzyme 5. Cat No: EXWM-0661. | |
| tRNAPhe [7-(3-amino-3-carboxypropyl)wyosine37-O]-methyltransferase | The enzyme is found only in eukaryotes, where it is involved in the biosynthesis of wybutosine, a hypermodified tricyclic base found at position 37 of certain tRNAs. The modification is important for translational reading-frame maintenance. In some species that produce hydroxywybutosine the enzyme uses 7-(2-hydroxy-3-amino-3-carboxypropyl)wyosine37 in tRNAPhe as substrate. The enzyme also has the activity of EC 2.3.1.231, tRNAPhe 7-[(3S)-4-methoxy-(3-amino-3-carboxypropyl)wyosine37-O]-carbonyltransferase. Group: Enzymes. Synonyms: TYW4 (ambiguous); tRNA-yW synthesizing enzyme-4 (ambiguous). Enzyme Commission Number: EC 2.1.1.290. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-1897; tRNAPhe [7-(3-amino-3-carboxypropyl)wyosine37-O]-methyltransferase; EC 2.1.1.290; TYW4 (ambiguous); tRNA-yW synthesizing enzyme-4 (ambiguous). Cat No: EXWM-1897. | |
| tRNAPhe {7-[3-amino-3-(methoxycarbonyl)propyl]wyosine37-N}-methoxycarbonyltransferase | The enzyme is found only in eukaryotes, where it is involved in the biosynthesis of wybutosine, a hypermodified tricyclic base found at position 37 of certain tRNAs. The modification is important for translational reading-frame maintenance. In some species that produce hydroxywybutosine the enzyme uses 7-[2-hydroxy-3-amino-3-(methoxycarbonyl)propyl]wyosine37 in tRNAPhe as substrate. The enzyme also has the activity of EC 2.1.1.290, tRNAPhe [7-(3-amino-3-carboxypropyl)wyosine37-O]-methyltransferase. Group: Enzymes. Synonyms: TYW4 (ambiguous); tRNA-yW synthesizing enzyme-4 (ambiguous). Enzyme Commission Number: EC 2.3.1.231. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-2179; tRNAPhe {7-[3-amino-3-(methoxycarbonyl)propyl]wyosine37-N}-methoxycarbonyltransferase; EC 2.3.1.231; TYW4 (ambiguous); tRNA-yW synthesizing enzyme-4 (ambiguous). Cat No: EXWM-2179. | |
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