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ATTO 590 is a novel fluorescent label belonging to the class of Rhodamine dyes. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. The ATTO dyes are a series of fluorescent dyes that provide all the crucial properties required for modern fluorescent technologies, such as fluorescence microscopy, flow-cytometry, fluorescence in situ hybridization (FISH), receptor binding assays or enzyme assays. The dye is highly suitable for single-molecule detection applications and high-resolution microscopy. The NHS-esters are used in common conjugation protocols.ATTO 590 is a novel fluorescent label belonging to the class of Rhodamine dyes, which has an absorption maximum of 594 nm and an emission maximum of 624 nm. ATTO 590-N-hydroxysuccinimide (NHS) is membrane permeable. Uses: Molar absorption 120,000 1/m cm, abs: 593 nm, em: 620 nm, qy=0.93, tfl 4.0 ns (unpublished data)atto 590 nhs ester may be suitable for use in site-specific labeling of human embryonic kidney (hek293t) cell lysates for western blotting, fluorescence, and widefield microscopy studies. Synonyms: 2-(6, 20-diethyl-7, 7, 9, 17, 19, 19-hexamethyl-2-oxa-20-aza-6-azoniapentacyclo[12.8.0.03, 12.05, 10.016, 21; docosa-1(14),3,5,8,10,12,15,17,21-nonaen-13-yl)-4-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoic acid;perchlorate; 6-(2-Carboxy-5-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}phenyl)-1,11-diethyl-2,2,4,8,10,10-hexamethyl-10,11-dihydro-2H-pyrano[3,2-g:5,6-g']diquinolin-1-ium perchlorate. Grades: ≥60% (coupling to amines). CAS No. 670269-33-7. Molecular formula: C41H42ClN3O11. Mole weight: 788.24.
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