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The enzyme, characterized from the bacterium Pseudomonas sp. CBB1, is part of the bacterial C-8 oxidation-based caffeine degradation pathway. The product decomposes spontaneously to a racemic mixture of 3,6,8-trimethylallantoin. The enzyme shows no acitivity with urate. cf. EC 1.14.13.113, FAD-dependent urate hydroxylase. Group: Enzymes. Synonyms: tmuM (gene name). Enzyme Commission Number: EC 1.14.13.212. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-0814; 1,3,7-trimethyluric acid 5-monooxygenase; EC 1.14.13.212; tmuM (gene name). Cat No: EXWM-0814.
7-methylxanthine demethylase
A non-heme iron oxygenase. The enzyme from the bacterium Pseudomonas putida prefers NADH over NADPH. The enzyme is specific for 7-methylxanthine. Forms part of the caffeine degradation pathway. Group: Enzymes. Synonyms: ndmC (gene name). Enzyme Commission Number: EC 1.14.13.128. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-0728; 7-methylxanthine demethylase; EC 1.14.13.128; ndmC (gene name). Cat No: EXWM-0728.
7-methylxanthosine synthase
The enzyme is specific for xanthosine, as XMP and xanthine cannot act as substrates. The enzyme does not have N1- or N3- methylation activity. This is the first methylation step in the production of caffeine. Group: Enzymes. Synonyms: xanthosine methyltransferase; XMT; xanthosine:S-adenosyl-L-methionine methyltransferase; CtCS1; CmXRS1; CaXMT1; S-adenosyl-L-methionine:xanthosine 7-N-methyltransferase. Enzyme Commission Number: EC 2.1.1.158. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-1754; 7-methylxanthosine synthase; EC 2.1.1.158; xanthosine methyltransferase; XMT; xanthosine:S-adenosyl-L-methionine methyltransferase; CtCS1; CmXRS1; CaXMT1; S-adenosyl-L-methionine:xanthosine 7-N-methyltransferase. Cat No: EXWM-1754.
caffeine dehydrogenase
This enzyme, characterized from the soil bacterium Pseudomonas sp. CBB1, catalyses the incorporation of an oxygen atom originating from a water molecule into position C-8 of caffeine. The enzyme utilizes short-tail ubiquinones as the preferred electron acceptor. Group: Enzymes. Enzyme Commission Number: EC 1.17.5.2. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-1095; caffeine dehydrogenase; EC 1.17.5.2. Cat No: EXWM-1095.
caffeine synthase
Paraxanthine is the best substrate for this enzyme but the paraxanthine pathway is considered to be a minor pathway for caffeine biosynthesis. Group: Enzymes. Synonyms: dimethylxanthine methyltransferase; 3N-methyltransferase; DXMT; CCS1; S-adenosyl-L-methionine:3,7-dimethylxanthine 1-N-methyltransferase. Enzyme Commission Number: EC 2.1.1.160. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-1757; caffeine synthase; EC 2.1.1.160; dimethylxanthine methyltransferase; 3N-methyltransferase; DXMT; CCS1; S-adenosyl-L-methionine:3,7-dimethylxanthine 1-N-methyltransferase. Cat No: EXWM-1757.
methylxanthine N3-demethylase
A non-heme iron oxygenase. The enzyme from the bacterium Pseudomonas putida shares an NAD(P)H-FMN reductase subunit with EC 1.14.13.178, methylxanthine N1-demethylase, and has higher activity with NADH than with NADPH. Also demethylates caffeine and theophylline with lower efficiency. Forms part of the degradation pathway of methylxanthines. Group: Enzymes. Synonyms: ndmB (gene name). Enzyme Commission Number: EC 1.14.13.179. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-0777; methylxanthine N3-demethylase; EC 1.14.13.179; ndmB (gene name). Cat No: EXWM-0777.
N-methyl nucleosidase
The enzyme preferentially hydrolyses 3- and 7-methylpurine nucleosides, such as 3-methylxanthosine, 3-methyladenosine and 7-methylguanosine. Hydrolysis of 7-methylxanthosine to form 7-methylxanthine is the second step in the caffeine-biosynthesis pathway. Group: Enzymes. Synonyms: 7-methylxanthosine nucleosidase; N-MeNase; N-methyl nucleoside hydrolase; methylpurine nucleosidase. Enzyme Commission Number: EC 3.2.2.25. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-3979; N-methyl nucleosidase; EC 3.2.2.25; 7-methylxanthosine nucleosidase; N-MeNase; N-methyl nucleoside hydrolase; methylpurine nucleosidase. Cat No: EXWM-3979.
Tannase (Food grade)
Tannase is an enzyme that enables the production of clear tea beverages while maintaining the tea's original deep flavor. The "browning" of tea beverages occurs because the caffeine and catechins in the tea create compounds that do not dissolve in cold water. Tannase separates the gallic acid (which is related to the bonding of caffeine and catechin) from the catechins, thus preventing the browning effect. Applications: Tannins enzymes widely used in food processing, feed processing, cosmetics and leather production process. Group: Enzymes. Synonyms: tannase; 9025-71-2; Tannin acyl Hydrolase. CAS No. 9025-71-2. Tannase. Activity: 300 Units/g. Appearance: Light-brown Powder. Storage: Sealed, dark, below 25°C. Source: Aspergillus oryzae. tannase; 9025-71-2; Tannin acyl Hydrolase. Cat No: TAC-3000.
theobromine synthase
This is the third enzyme in the caffeine-biosynthesis pathway. This enzyme can also catalyse the conversion of paraxanthine into caffeine, although the paraxanthine pathway is considered to be a minor pathway for caffeine biosynthesis. Group: Enzymes. Synonyms: monomethylxanthine methyltransferase; MXMT; CTS1; CTS2; S-adenosyl-L-methionine:7-methylxanthine 3-N-methyltransferase. Enzyme Commission Number: EC 2.1.1.159. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-1755; theobromine synthase; EC 2.1.1.159; monomethylxanthine methyltransferase; MXMT; CTS1; CTS2; S-adenosyl-L-methionine:7-methylxanthine 3-N-methyltransferase. Cat No: EXWM-1755.
Caffeine, Laboratory Grade, 25 g
Formula: C8H10N4O2. Formula Wt: 194. 19CAS: 58-08-2 Characteristic: White powder. Storage Code: Green; general chemical storage. Notes: Naturally occurs in tea, coffee, guarana paste, and cola nuts; light sensitive. Alternative Names: No-Doz. Grades: chem-grade laboratory. Product ID: 851568. -- SOLD FOR EDUCATIONAL USE ONLY --
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