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| Product | Description | |
|---|---|---|
| 2×HiFi PCR SuperMix | 2×HiFi PCR SuperMix. 2×hifi pcr supermix is a ready-to-use mixture of hifi dna polymerase dna polymerase, dntps and optimized buffer. 2×hifi pcr supermix is optimized for the amplification of genomic dna and pcr supermix ii is optimized for the amplification of λdna, cdna or plasmid dna. the supermix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and h2o. Group: Cloning Enzymes. Purity: 1ml; 5ml. Storage: Store at -20 ?. Cat No: CE-3021. |  |
| 2×HiFi Pfu PCR SuperMix (-dye ) | 2×HiFi Pfu PCR SuperMix (-dye ). 2×hifi pfu pcr supermix (-dye ) is a ready-to-use mixture of hifi pfu dna polymerase, dntps and optimized buffer. the supermix is provided at 2× concentration and used at 1× concentration by adding template, primers and h2o. Group: Cloning Enzymes. Purity: 1ml; 5ml. Storage: Store at -20 ?. Cat No: CE-3002. | |
| 2×KD Plus PCR SuperMix | 2×KD Plus PCR SuperMix. 2×kd plus pcr supermix is a ready-to-use mixture of kd plus dna polymerase, dntps, and optimized buffer. the supermix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and h2o. Group: Cloning Enzymes. Purity: 1ml; 5ml. Storage: Store at -20 ?. Cat No: CE-3020. | |
| 2×Pfu PCR SuperMix (-dye ) | 2×Pfu PCR SuperMix (-dye ). 2×pfu pcr supermix (-dye ) is a ready-to-use mixture of pfu dna polymerase, dntps, and optimized buffer. the supermix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and h2o. Group: Cloning Enzymes. Purity: 1ml; 5ml. Storage: Store at -20 ?. Cat No: CE-3018. | |
| 2×Speed Pfu PCR SuperMix (-dye ) | 2×Speed Pfu PCR SuperMix (-dye ). Transstart® fastpfu fly pcr supermix is a ready-to-use mixture of transstart® fastpfu fly dna polymerase, dntps, and optimized buffer. the supermix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and h2o.; transstart® fastpfu fly pcr supermix offers 108-fold fidelity as compared to easytaq® dna polymerase. extension rate is about 2-6 kb/min. pcr products can be directly cloned into peasy®-blunt vectors. amplification of genomic dna fragment up to 15 kb. amplification of plasmid dna fragment up to 20 kb. Group: Cloning Enzymes. Purity: 1ml; 5ml; 15ml. Storage: Store at -20 ?. Cat No: CE-3017. | |
| 2×TA PCR SuperMix | 2×TA PCR SuperMix. 2×ta pcr supermix is a ready-to-use mixture of ta dna polymerase, dntps and optimized buffer. the supermix is provided at 2× concentration and used at 1× concentration by adding template, primers and h2o. efficiency of pcr products with a is equal to taq dna polymerase. it is more suitable for high fidelity ta cloning. Group: Cloning Enzymes. Purity: 1ml; 5ml. Storage: Store at -20 ?. Cat No: CE-3023. | |
| 2×Taq PCR SuperMix | 2×Taq PCR SuperMix. 2×taq pcr supermix is a ready-to-use mixture of taq dna polymerase, dntps and optimized buffer. the supermix is provided at 2×concentration and used at 1× concentration by adding template, primers and h2o. pcr products are not suitable for directly load to page. Group: Cloning Enzymes. Purity: 1ml; 5ml; 15ml. Storage: Store at -20 ?. Cat No: CE-3005. | |
| 2×Taq PCR SuperMix for PAGE(+dye) | 2×Taq PCR SuperMix for PAGE(+dye). 2×taq pcr supermix for page(+dye) is a ready-to-use mixture of dna polymerase for page, dntps and optimized buffer. the supermix for page is provided at 2× concentration and used at 1× concentration by adding template, primers and h2o. Group: Cloning Enzymes. Purity: 1ml; 5ml; 15ml. Storage: Store at -20 ?. Cat No: CE-3006. | |
| 3'-(6-Fluorescein) Frits column (100nmol) | 3'-(6-Fluorescein) Frits column (100nmol), an integral equipment utilized in the dynamic and intricate biomedicine sector to purify DNA. With superior capacity and groundbreaking technology, this top-tier product capacitates swift and dependable removal of unwarranted impurities from DNA samples, positioning it optimally for downstream applications such as sequencing, PCR, and cloning. Synonyms: 3'-(6-Fluorescein) Frits column. |  |
| 3'-Deoxythymidine-5'-triphosphate lithium salt | 3'-Deoxythymidine-5'-triphosphate lithium salt, an indispensable entity in the realm of biomedicine, constitutes a pivotal building block. Within molecular biology research, it assumes multifaceted roles, serving as both a DNA polymerase substrate and a nucleotide analog. Profoundly contributing to the study of DNA sequencing, gene cloning, and gene expression analysis, this particular product also bears significance in crafting therapeutic approaches for maladies such as viral infections and cancers. Synonyms: dTTP; sodium ((2S,5R)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl trihydrogentriphosphate. Grades: ≥ 95%. CAS No. 128524-26-5. Molecular formula: C10H17N2O13P3·xLi. Mole weight: 466.17 (free acid). | |
| Antarctic Phosphatase from E. coli, Recombinant | Antarctic Phosphatase catalyzes the removal of 5? phosphate from DNA and RNA. Since phosphatase-treated fragments lack the 5? phosphoryl termini required by ligases, they cannot self-ligate. This property can be used to decrease the vector background in cloning strategies. Applications: Removing 5? phosphates from dna, rna, rntps and dntps preparation of templates for 5? end labeling prevention of recircularization of cloning vectors removal of dntps and pyrophosphate from pcr reactions dephosphorylation of proteins. Group: Enzymes. Synonyms: Antarctic Phosphatase. Purity: >95% estimated by SDS-PAGE. Antarctic Phosphatase. Mole weight: Apparent: 35 kDa Theoretical: 69 kDa. Storage: at -20°C. Form: 10 mM Tris-HCl (pH 7.4), 1 mM MgCl2, 0.01 mM ZnCl2, 1 mM DTT and 50% glycerol. Source: E. coli. Species: E. coli. Antarctic Phosphatase. Cat No: NATE-1399. | |
| Blood PCR Kit | Blood PCR Kit. Blood pcr kit is designed for dna amplification from whole blood without dna extraction. Group: Cloning Enzymes. Purity: 100×; 500×. Storage: Store at -20 ?. Cat No: CE-3012. | |
| Cas9-C-NLS Nuclease | Cas9-C-NLS Nuclease. Cas9-c-nls nuclease is the recombinant streptococcus pyogenes cas9 (wt) protein with a c-terminal nucleic localization signal (nls). it can be used for genome editing by inducing site-specific dna double stranded breaks. cas9 protein forms a very stable ribonucleoprotein (rnp) complex with the guide rna (grna) component of the crispr/cas9 system, which can localize to the nucleus immediately once entering the cell with the guide of the nls. compared with the mrna or plasmid systems, transcription and translation processes are not required. this dna-free system avoids the risk of inserting foreign dna into the genome, which can be quite useful for gene editing-based disease therapy. our highly pure and active cas9 nuclease meets all of the researchers requirements (e.g. in vitro cleavage assay, rnp complex transfection, micro injection). Group: Cloning Enzymes. Purity: 50μg; 100μg. Storage: Store at -20 ?. Source: E.coli. Pack: 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol PH 7.4 at 25°C. Cat No: CE-3502. | |
| Cas9-N-NLS Nuclease | Cas9-N-NLS Nuclease. Cas9-n-nls nuclease is the recombinant streptococcus pyogenes cas9 (wt) protein with a n-terminal nucleic localization signal (nls) that can be used for genome editing by inducing site-specific dna double stranded breaks. cas9 protein forms a very stable ribonucleoprotein (rnp) complex with the guide rna (grna) component of the crispr/cas9 system, which can localize to the nucleus immediately once entering the cell with the guide of the nls. compared with the mrna or plasmid systems, transcription and translation processes are not required. this dna-free system avoids the risk of inserting foreign dna into the genome, which can be quite useful for gene editing-based disease therapy. our highly pure and active cas9 nuclease meets all of the researcher´s requirements (e.g. in vitro cleavage assay, rnp complex transfection, micro injection). Group: Cloning Enzymes. Purity: 50μg; 100μg. Storage: Store all components at -20 ?. Source: E.coli. Cat No: CE-3503. | |
| Cas9 Nuclease | Cas9 Nuclease. Recombinant streptococcus pyogenes cas9 (wt) nuclease is purified from e. coli.it can be used for genome editing by inducing site-specific double stranded breaks in double stranded dna. cas9 protein forms a very stable ribonucleoprotein (rnp) complex with the guide rna (grna) component of the crispr/cas9 system. the rnp complex recognizes the target site by matching grna with the genomic dna sequence and leads to dna breaks within 3 bases from the ngg pam (protospacer adjacent motif). with gencrispr cas9 nuclease, customers can screen highly efficient grna using in vitro dna cleavage assays. it could be a powerful tool for gene editing. Group: Cloning Enzymes. Purity: 10μg; 50μg. Storage: Store at -20 ?. Source: E.coli. Cat No: CE-3501. | |
| DNA Polymerase for PAGE | DNA Polymerase for PAGE. Dna polymerase for page is purified from e. coliexpressing a cloned dna polymerase from thermus aquaticus. the enzyme consists of a single polypeptide with a molecular weight of approximately 94 kda. dna polymerase for page has 5?-3? dna polymerase activity and 5?-3? exonuclease activity. lt lacks 3?-5? exonuclease activity. this enzyme is supplied with unique buffer, and its pcr product is suitable for sds-page and agarose gel electrophoresis. Group: Cloning Enzymes. Purity: 2500U. Storage: Store at -20 ?. Cat No: CE-3004. | |
| GC Enhancer | GC Enhancer. Gc enhancer should be used with dna ploymerase to optimize pcr from complex templates including gc rich. the storage concentration is 10×. the working concentration can adjust between 0.5×-5×. Group: Cloning Enzymes. Purity: 200ul. Storage: Store at -20 ?. Cat No: CE-3007. | |
| HiFi DNA Polymerase | HiFi DNA Polymerase. Hifi dna polymerase contains ta dna polymerase and a proofreading 3-5 exonuclease.hifi dna polymerase provides higher specificity and higher amplification efficiency than ta dna polymerase. two different buffers are provided in the kit. hifi pcr buffer i is optimized for the amplification of genomic dna and hifi pcr buffer ii is optimized for the amplification of λdna, cdna or plasmid dna. extension rate is about 1-2 kb/min. template-independent a can be generated at the 3 end of the pcr product. amplification of genomic dna fragment up to 15 kb. Group: Cloning Enzymes. Purity: 250U; 500U. Storage: Store at -20 ?. Cat No: CE-3022. | |
| HiFi Pfu DNA Polymerase | HiFi Pfu DNA Polymerase. Hifi pfu dna polymerase is an engineered version of pfu dna polymerase with enhanced yield and higher fidelity. ehifi pfu dna polymerase possesses a proofreading 3'-5' exonuclease activity. hifi pfu dna polymerase offers high fidelity. extension rate is about 0.5 kb/min. amplification of genomic dna fragment up to 6 kb. amplification of plasmid dna fragment up to 10 kb. Group: Cloning Enzymes. Purity: 250U; 500U. Storage: Store at -20 ?. Cat No: CE-3001. | |
| High Pure dNTPs | High Pure dNTPs. High pure dntps is an equal molar solution of high quality datp, dctp, dgtp, and dttp with purity up to 99%. it is suitable for. pcr, qpcr, dna sequencing and cdna synthesis. Group: Cloning Enzymes. Purity: 1ml; 5ml. Storage: Store at -20 ?. Cat No: CE-3008. | |
| IPTG Dioxane Free, Sterile Solution, 1M | Sterile solution of dioxane-free IPTG manufactured according to Maniatas, et al. Suitable for use in molecular cloning and M13 bacteriophage plating. Prepared with sterile, RNase and DNase-free molecular biology grade water and ultrapure RNase and DNase-free IPTG.IPTG is a carbohydrate used to induce b-galactosidase in the selection of recombinant plasmids. Used to select for lac Y mutants and to induce the lac operon in E.coli. IPTG will induce the cellular content of lactose permease. Water:Distilled, deionized waterFiltration: Sterile filtered (0.2um)IPTG Component: ≥99%Dioxane: ≤ 0.001%RNase, DNase, Proteases: None Detected. Group: Biochemicals. Alternative Names: IPTG; Isopropyl-ß-D-thiogalactopyranoside; Isopropyl-ß-D-thiogalactoside. Grades: Molecular Biology Grade. CAS No. 367-93-1. Pack Sizes: 10ml, 5x10ml, 100ml. Molecular Formula: C9H18O5S, Molecular Weight: 238.31. US Biological Life Sciences. |  Worldwide |
| KD Plus DNA Polymerase | KD Plus DNA Polymerase. Kd plus dna polymerase is a genetically modified high fidelity dna polymerase. this enzyme provides higher amplification capability than traditional pfu dna polymerase and fast amplification speed equal to taq dna polymerase (1 kb/min). due to strong 3-5 exonuclease activity, this enzyme offers 108-fold fidelity as compared to taq dna polymerase. amplification of genomic dna fragment up to 15 kb. amplification of plasmid dna fragment up to 20 kb. Group: Cloning Enzymes. Purity: 100U; 200U. Storage: Store at -20 ?. Cat No: CE-3019. | |
| Mouse Genotyping Kit | Mouse Genotyping Kit. Mouse genotyping kit uses a unique lysis buffer to prepare mouse genotyping pcr-ready dna from fresh or frozen mouse tissue slices, such as mouse ears, toes and tails. Group: Cloning Enzymes. Purity: 100×; 500×. Storage: Store at -20 ?. Cat No: CE-3013. | |
| Mutation Detection Kit | Mutation Detection Kit. Mutation detection kit provides a simple, reliable, and rapid method for the detection of site specific cleavage of genomic dna that is extracted from cells transfected with constructs expressing engineered nucleases such as transcription activator-like effector nucleases (talen), clustered regularly interspaced short palindromic repeats (crispr)/cas9, or zinc-finger nucleases (zfn). mutation detection kit includes high-fidelity dna polymerase for amplifying the target regions from cells, and t7 endonuclease i for recognizing and detecting the mismatches caused by gene editing tools. it provides an easy and reliable approach for estimating the efficiency of genome editing. components. high-fidelity dna polymerase. 5x pcr reaction buffer. t7 endonuclease i. 10x gencrispr t7 endonuclease i reaction buffer. control template dna. control primer mix. protease k. dntp. Group: Cloning Enzymes. Purity: 25 reactions/kit. Storage: Store at -20 ?. Source: E.coli. Pack: 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.4, at 25°C. Cat No: CE-3508. | |
| Native Avian myeloblastosis virus AMV-Reverse Transcriptase | A Reverse transcriptase (RT) is an enzyme used to geneRate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription. It is mainly associated with retroviruses. However, non-retroviruses also use RT (for example, the hepatitis B virus, a member of the Hepadnaviridae, which are dsDNA-RT viruses, while retroviruses are ssRNA viruses). RT inhibitors are widely used as antiretroviral drugs. RT activities are also associated with the replication of chromosome ends (telomerase) and some mobile genetic elements (retrotransposons). Applications: Amv reverse transcriptase synthesizes dna complementary (cdna) to rna templates. a dna primer complementary to the rna template and a divalent cation, either mg or mn, are required for initiation of transcription. this enzyme is commonly used to make cdnas from mrna for eventual cloning or for use as probes. Group: Enzymes. Synonyms: DNA nucleotidyltransferase (RNA-directed); reverse transcripta. Enzyme Commission Number: EC 2.7.7.49. CAS No. 9068-38-6. RT. Storage: -70°C. Source: Avian myeloblastosis virus. DNA nucleotidyltransferase (RNA-directed); reverse transcriptase; revertase; RNA-dependent deoxyribonucleate nucleotidyltransferase; RNA revertase; RNA-dependent DNA polymerase; RNA-instructed DNA polymerase; RT; EC 2.7.7.49; 9068-38-6. Cat No: NATE-0073. | |
| NLS-Cas9-D10A Nickase | NLS-Cas9-D10A Nickase. Nls-cas9-d10a nickase is a mutation form of cas9 nuclease which makes one active domain deactivated, thus it can only cut one single strand dna that is complementary to the guide-rna, producing one single strand cut. combined with two different grna, nls-cas9-d10a nickase produces two cut sites respectively and causes a double strand break. compared with the wild type cas9 nuclease, the two-grna guided cleavage can significantly reduce the off target effects. Group: Cloning Enzymes. Purity: 10μg; 50μg. Storage: Store at -20 ?. Source: E.coli. Pack: 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.4 at 25 °C. Cat No: CE-3505. | |
| NLS-Cas9-NLS Nuclease | NLS-Cas9-NLS Nuclease. Nls-cas9-nls is produced by expression in an e. coli strain carrying a plasmid encoding the cas9 gene from streptococcus pyogenes with a double-ends nuclear localization signal (nls). it can be used for genome editing by inducing site-specific dna double stranded breaks. cas9 protein forms a very stable ribonucleoprotein (rnp) complex with the guide rna (grna) component of the crispr/cas9 system, which can localize to the nucleus immediately once entering the cell with the guide of the nls. compared with the mrna or plasmid systems, transcription and translation processes are not required. this dna-free system avoids the risk of inserting foreign dna into the genome, which can be quite useful for gene editing-based disease therapy. our highly pure and active cas9 nuclease meets all of the researcher´s requirements (e.g. in vitro cleavage assay, rnp complex transfection, micro injection). Group: Cloning Enzymes. Purity: 50μg; 100μg. Storage: Store at -20 ?. Source: E.coli. Pack: 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.4 at 25?. Cat No: CE-3504. | |
| Nucleoside Deoxyribosyltransferase II from Lactobacillus leichmanii, Recombinant | Class II N-Deoxyribosyltranferases, DRTases, catalyze the transfer of a 2'-deoxyribosyl group between purines or pyrimidines. In the absence of an acceptor nucleobase, these enzymes display hydrolase activity, converting the nucleoside to its base and a deoxyribose. In lactobacilli species, Nucleoside Deoxyribosyltransferase enzymes are part of the nucleoside salvage pathway for DNA synthesis. Applications: Nucleoside deoxyribosyltransferase ii has been used in a study that assessed its enzymatic synthesis with 2?-deoxyguanosine. nucleoside deoxyribosyltransferase ii has also been used in studies to investigate its molecular cloning, expression and spe... 2.4.2.6. CAS No. 9026-86-2. NDT. Storage: -20°C. Form: lyophilized powder. Source: E. coli. Species: Lactobacillus leichmanii. EC 2.4.2.6; purine (pyrimidine) nucleoside:purine (pyrimidine) deoxyribosyl transferase; deoxyribose transferase; nucleoside trans-N-deoxyribosylase; trans-deoxyribosylase; trans-N-deoxyribosylase; trans-N-glycosidase; nucleoside deoxyribosyltransferase I (purine nucleoside:purine deoxyribosyltransferase:strictly specific for transfer between purine bases); nucleoside deoxyribosyltransferase II [purine (pyrimidine) nucleoside:purine (pyrimidine) deoxyribosyltransferase]; DRTase; Deoxyribose transferase; NDT. Cat No: NATE-0478. | |
| PC Spacer Phosphoramidite | PC Spacer phosphoramidite can be used as an intermediate to link any modification reagent (which can be used as phosphoramidite) to the end of the oligonucleotide. After light cleavage, 5'-phosphate is generated on the DNA, making it suitable for further biotransformation, such as gene construction and cloning after ligation. Synonyms: [4-(4,4'-Dimethoxytrityloxy)butyramidomethyl)-1-(2-nitrophenyl)-ethyl]-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite. Molecular formula: C43H53N4O8P. Mole weight: 784.88. | |
| Pfu DNA Polymerase | Pfu DNA Polymerase. Pfu dna polymerase is a fast, high fidelity and high processivity hot start dna polymerase. extension rate is about 2-4 kb/min. amplification of genomic dna fragment up to 15 kb. amplification of plasmid dna fragment up to 20 kb. Group: Cloning Enzymes. Purity: 250U; 500U. Storage: Store at -20 ?. Cat No: CE-3015. | |
| Plant Tissue PCR Kit | Plant Tissue PCR Kit. Plant tissue pcr kit uses a unique lysis buffer to lyse plant tissues (fresh or frozen). the resulting lysate without purification can be directly used as pcr template. pcr product can be directly used for gel electrophoresis. dna fragment up to 2 kb and can be used for plants with moderate contents of polysaccharides or polyphenols. Group: Cloning Enzymes. Purity: 100×; 500×. Storage: Store at -20 ?. Cat No: CE-3014. | |
| Reverse Transcriptase from HIV, Recombinant | Chromatographically purified heterodimer composed of 66kDa and 51kDa subunits. Supplied as a solution in 10mM potassium phosphate, pH 7.4, 1mM DTT and 20% glycerol. Primarily for AIDS research purposes; this enzyme has less fidelity than the AMV enzyme in other applications such as the preparation of cDNA from mRNA for cloning purposes. Applications: Hiv reverse transcriptase is used for research on the aids primer. however it can be substituted for amv reverse transcriptase, which is mainly used to transcribe mrna into double stranded cdna, that can be inserted into prokaryotic vectors. the enzyme can also be used with either single stranded dna or rna templates to mak...or labeling the termini of dna fragments with protruding 5' termini. the enzyme can also be used to sequence dnas by the dideoxy chain termination method of sanger when the klenow fragment of e. coli dna polymerase i, or the t7 dna polymerase yield unsatisfactory results. Group: Enzymes. Synonyms: Reverse transcriptase; RT. Enzyme Commission Number: EC 2.7.7.49. CAS No. 9068-38-6. Purity: Chromatographically purified. RT. Mole weight: 66 kDa and 51 kDa. Activity: > 5,000 units per mg protein. Storage: Store at -20°C. Form: A solution in 10mM potassium phosphate, pH 7.4, 1mM DTT and 20% glycerol. Source: E. coli. Species: HIV. Reverse transcriptase; RT. Cat No: NATE-0987. | |
| sgRNA Screening Kit | sgRNA Screening Kit. Sgrna screening kit provides a simple, reliable, and rapid method for assessing sgrna efficiency before cell transduction, allowing you to identify the highly effective crispr sgrna. cas9 nuclease nls is an rna-guided endonuclease that catalyzes site-specific cleavage of double stranded dna. the location of the break is within the target sequence 3 bases from the ngg pam (protospacer adjacent motif). the design of single guide rna (sgrna) is dependent on the target region close to the pam site. even if you pick a target sequence that fulfills all of the described requirements, sgrna specificity and activity is unpredictable. therefore, it is often recommended that multiple, different sgrnas be designed to target a gene of interest. components. gencrispr cas9 nuclease. 10x reaction buffer. positive control sgrna. positive control substrate. rnase-free water. Group: Cloning Enzymes. Purity: 30 reactions/kit. Storage: Store at -20 ?. Source: E.coli. Pack: 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol PH 7.4, at 25°C. Cat No: CE-3509. | |
| sgRNA Synthesis Kit | sgRNA Synthesis Kit. This product is designed to synthesize grnas in vitro. in the bacterial crispr/cas9 system, the cas9 nuclease associates with two rnas, the crispr rna (crrna) and the trans-activating crrna (tracrrna), to direct sequence-specific dna cleavage. the grna (guide rna) is a fusion of the natural crrna and tracrrna components. it contains an 18-20 base variable sequence that can be changed to target any dna sequence that is adjacent to an ngg proto-spacer adjacent motif (pam) on the 3 end of the target sequence. the sgrna synthesis kit allows user to fuse their choice of 18-20 base target sequence within the grna dna sequence provided, by pcr fusion. the kit also provides materials for in-vitro transcription of the generated grna dna in order to generate grnas which can be directly used for in-vivo genome editing. Group: Cloning Enzymes. Purity: 20 reactions/kit. Storage: Store at -20 ?. Source: E.coli. Pack: 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol PH 7.4, at 25°C. Cat No: CE-3510. | |
| Speed Pfu DNA Polymerase | Speed Pfu DNA Polymerase. Speed pfu dna polymerase is a hot start, high fidelity and high processivity dna polymerase. speed pfu dna polymerase has an extension rate of up to 6 kb/min. speed pfu dna polymerase has high extension rate, fidelity, and amplification efficiency. extension rate is about 2-6 kb/min. amplification of genomic dna fragment up to 15 kb. amplification of plasmid dna fragment up to 20 kb. Group: Cloning Enzymes. Purity: 250U; 500U. Storage: Store at -20 ?. Cat No: CE-3016. | |
| TA DNA Polymerase | TA DNA Polymerase. Ta dna polymerase is a mixture of taq dna polymerase with a proofreading 3-5 exonuclease. the fidelity is equal to hifi pfu dna polymerase. the yield is equal to that from taq dna polymerase. it is more suitable for high fidelity ta cloning. ta dna polymerase offers 18-fold fidelity as compared to taq dna polymerase. extension rate is about 1-2 kb/min. template-independentacan be generated at the 3end of the pcr product. amplification of genomic dna fragment up to 8 kb. Group: Cloning Enzymes. Purity: 250U; 500U. Storage: Store at -20 ?. Cat No: CE-3024. | |
| Taq DNA Polymerase | Taq DNA Polymerase. Taq dna polymerase is purified from e. coli expressing a cloned dna polymerase from thermus aquaticus. the enzyme consists of a single polypeptide with a molecular weight of approximately 94 kda. taq dna polymerase has 5?-3? dna polymerase activity and 5?-3? exonuclease activity. it lacks 3?-5? exonuclease activity. taq dna polymerase is suitable for routine amplification. pcr products are unsuitable for page. extension rate is about 1-2 kb/min. template-independent a can be generated at the 3? end of the pcr product. amplification of genomic dna fragment up to 4 kb. Group: Cloning Enzymes. Purity: 500U; 2500U. Storage: Store at -20 ?. Cat No: CE-3003. | |
| Thymidine 5'-triphosphate-3'-monophosphate | Thymidine 5'-triphosphate-3'-monophosphate, renowned for its indispensable role in DNA synthesis and DNA-based applications, assumes a paramount position within the biomedical sector. Revered as a pivotal precursor of DNA, this product proficiently facilitates a myriad of molecular biology techniques, including but not limited to PCR amplification, DNA sequencing, and cloning. Furthermore, it engenders invaluable assistance in unraveling the complexities of DNA replication, repair, and transcription, thereby contributing significantly to the realm of genetic disease and cancer investigations and therapeutics. CAS No. 1015762-49-8. Molecular formula: C10H18N2O17P4. Mole weight: 562.15. | |
| Aat II | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 10-fold overdigestion with enzyme about 90% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 500U; 2500U. GACGT↑C C↓TGCAG. Activity: 20000u.a./ml. Appearance: 10X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: An E.coli strain, that carries the cloned Aat II gene from Acetobacter aceti. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol. Cat No: RE-1001EN. | |
| Acu I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 2-fold overdigestion with enzyme about 80% of the dna fragments can be ligated. of these, 80% can be recut. Group: Restriction Enzymes. Purity: 50U; 250U. CTGAAG(N)16↑ GACTTC(N)14&darr. Activity: 1000u.a./ml. Appearance: 10 X SE-buffer Y, BSA, SAM. Storage: -20°C. Form: Liquid. Source: An. E.coli strain that carries the cloned Acu I gene from Acinetobacter calcoaceticus SRW4. Pack: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: RE-1013EN. | |
| Adenosine Kinase from Human, Recombinant | E.coli. Applications: Human adenosine kinase is an active and purified, 345-aa short form adk protein (39kda) cloned by rt-pcr amplification of mrna extracted from human hepatoma cells and expressed in e.coli. the sequence of the cloned adk (genbank accession number u50196) was confirmed by dna sequencing (100% identity). Group: Enzymes. Synonyms: ADK. Enzyme Commission Number: EC 2.7.1.20. CAS No. 9027-72-9. Mole weight: 39kDa. Activity: ≥ 0.200 unit/mg protein. Source: Human. Species: ADK. ADK; EC 2.7.1.20. Pack: stable lyophilized form. Cat No: NATE-1740. | |
| Afe I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (BamHI-digest) in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 10-fold overdigestion with enzyme more than 80% of dna pbr322 fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 200U; 1000U. AGC↑GCT TCG↓CGA. Activity: 10000u.a./ml. Appearance: 10 X SE-buffer B, BSA. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned Afe I gene from Alcaligenes faecalis T2774. Pack: 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: RE-1014EN. | |
| Alu I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 5-fold overdigestion with enzyme approximately 90% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 200U; 1000U. AG↑CT TC↓GA. Activity: 2000-5000u.a./ml. Appearance: 10 X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: An. E.coli strain that carries the cloned Alu I gene from Arthrobacter luteus. Pack: 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 200 μg/ml BSA; 1 mM DTT, 50% glycerol. Cat No: RE-1018EN. | |
| Apa I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-dcm-, BamHI-digest) in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 50-fold overdigestion with enzyme > 95% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 5000U; 25000U. GGGCC↑C C↓CCGGG. Activity: 50000u.a./ml. Appearance: 10 X SE-buffer Y, BSA. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned Apa I gene from Acetobacter pasteurianus. Pack: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Cat No: RE-1021EN. | |
| BamH I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 50-fold overdigestion with enzyme approximately 90% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 4000U; 20000U; 4000U; 20000U. G↑GATCC CCTAG↓G. Activity: 20000; 50000u.a./ml. Appearance: 10 X SE-buffer G, BSA. Storage: -20°C. Form: Liquid. Source: An E.coli strain, that carries the cloned gene BamHI from Bacillus amyloliquefaciens H. Pack: 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 100 μg/ml BSA; 1 mM DTT; 50% glycerol. Cat No: ET-1030RE. | |
| Bgl II | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 10-fold overdigestion with enzyme more than 90% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 1000U; 5000U. A↑GATCT TCTAG↓A. Activity: 10000u.a./ml. Appearance: 10 X SE-buffer O. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned Bgl II gene from Bacillus globigii. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1034RE. | |
| Bmt I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (Hind III-digest) in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 20-fold overdigestion with enzyme about 95% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 1000U; 5000U. GCTAG↑C C↓GATCG. Activity: 20000u.a./ml. Appearance: 10 X SE-buffer W. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned Bmt I gene from Bacillus megaterium S2. Pack: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1036RE. | |
| Cytosolic 5'-nucleotidase II from Huamn, Recombinant | E.coli. Applications: Human cytosolic imp/gmp specific 5'-nucleotidase/phosphotransferase ii (cn-II) is a pure and active protein of 65kda cloned by rt-pcr amplification of mrna extracted from human hepatoma cells and expressed in e.coli. the sequence of the cloned nt5c2 gene (genbank accession number p49902) was confirmed by dna sequencing (100% identity). Group: Enzymes. Synonyms: uridine 5'-nucleotidase; 5'-adenylic phosphatase; adenosine 5'-phosphatase; AMP phosphatase; adenosine monophosphatase; 5'-mononucleotidase; AMPase; UMPase; snake venom 5'-nucleotidase; thimidine monophosphate nucleotidase; 5'-AMPase; 5'-AMP nucleotidase; AMP phosphohydrolase; IMP 5'-nucleotidase; EC 3.. Enzyme Commission Number: EC 3.1.3.5. CAS No. 9027-73-0. AMPase. Mole weight: 65kDa. Activity: ≥ 0.150 unit/mg protein. Storage: -20 °C in a solution containing 50 mM Tris-HCl, pH 7.6, 2 mM β-mercaptoethanol, 50% glycerol. Source: Human. Species: cN-II. uridine 5'-nucleotidase; 5'-adenylic phosphatase; adenosine 5'-phosphatase; AMP phosphatase; adenosine monophosphatase; 5'-mononucleotidase; AMPase; UMPase; snake venom 5'-nucleotidase; thimidine monophosphate nucleotidase; 5'-AMPase; 5'-AMP nucleotidase; AMP phosphohydrolase; IMP 5'-nucleotidase; EC 3.1.3.5; CD73; NT5E; ecto-5'-nucleotidase. Pack: stable lyophilized form. Cat No: NATE-1742. | |
| Dna,(swine clone pmta1motilin[leu13]-specifying)(9ci) | Heterocyclic Organic Compound. CAS No. 116283-54-6. Molecular formula: C121H190N34O35. Mole weight: 2681.01. Purity: 0.96. IUPACName: [Leu13]motilin. Canonical SMILES: CCC (C)C (C (=O)NC (CC1=CC=CC=C1)C (=O)NC (C (C)O)C (=O)NC (CC2=CC=C (C=C2)O)C (=O)NCC (=O)NC (CCC (=O)O)C (=O)NC (CC (C)C)C (=O)NC (CCC (=O)N)C (=O)NC (CCCNC (=N)N)C (=O)NC (CC (C)C)C (=O)NC (CCC (=O)N)C (=O)NC (CCC (=O)O)C (=O)NC (CCCCN)C (=O)NC (CCC (=O)O)C (=O)NC (CCCNC (=N)N)C (=O)NC (CC (=O)N)C (=O)NC (CCCCN)C (=O)NCC (=O)NC (CCC (=O)N)C (=O)O)NC (=O)C3CCCN3C (=O)C (C (C)C)NC (=O)C (CC4=CC=CC=C4)N. Catalog: ACM116283546. |  |
| Dra III | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 10-fold overdigestion with enzyme 70% of the dna fragments can be ligated and recut. in the presence of 10%peg ligation is better. Group: Restriction Enzymes. Purity: 500 U; 2500U. CACNNN↑GTG GTG↓NNNCAC. Activity: 5000u.a./ml. Appearance: 10 X SE-buffer 2K, BSA. Storage: -20°C. Form: Liquid. Source: An E.coli strain, that carries the cloned gene Dra III from Deinococcus radiophilus. Pack: 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1097RE. | |
| EcoR I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 40-fold overdigestion with enzyme about 95% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 5000U; 25000U. G↑AATTC CTTAA↓G. Activity: 20000; 50000u.a./ml. Appearance: 10 X SE-buffer EcoRI, BSA. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned EcoR I gene from Escherichia coli. Pack: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1101RE. | |
| EcoR V | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 20-fold overdigestion with enzyme 90% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 2000U; 10000U. GAT↑ATC CTA↓TAG. Activity: 20000u.a./ml. Appearance: 10 X SE-buffer W, BSA. Storage: -20°C. Form: Liquid. Source: An E.coli strain, that carries the cloned gene EcoRV from Escherichia coli. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1102RE. | |
| FatI | One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 55°C in a total reaction volume of 50 μl. Applications: After 2-fold overdigestion with enzyme more than 90% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 100U; 500U. ↑CATG GTAC&darr. Activity: 5000u.a./ml. Appearance: 10 X SE-buffer G. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned Fat I gene from Flavobacterium aquatile NL3. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; and 50% glycerol. Cat No: ET-1108RE. | |
| Fau I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl. Applications: After 2-fold overdigestion with enzyme more than 90% of the dna fragments can be ligated and of these 95% can be recut. Group: Restriction Enzymes. Purity: 100U; 500U. CCCGC(N)4↑ GGGCG(N)6&darr. Activity: 2000u.a./ml. Appearance: 10 X SE-buffer B. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned Fau I gene from Flavobacterium aquatili. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1109RE. | |
| FauND I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 10-fold overdigestion with enzyme 80% of the dna fragments can be ligated and recut. in the presence of 10% peg ligation is better. Group: Restriction Enzymes. Purity: 1000U; 5000U. CA↑TATG GTAT↓AC. Activity: 10000u.a./ml. Appearance: 10 X SE-buffer Y, BSA. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned FauND I gene from Flavobacterium aquatili ND. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 1mM DTT; 200 μg/ml BSA, 50% glycerol. Cat No: ET-1110RE. | |
| Fbl I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl. Applications: After 2-fold overdigestion with enzyme approximately 90% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 100U; 500U. GT↑MKAC CAKM↓TG. Activity: 1000-2000u.a./ml. Appearance: 10 X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned Fbl I gene from Flavobacterium balustinum. Pack: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, and 50% glycerol. Cat No: ET-1111RE. | |
| FMN Reductase from Escherichia coli (Fre), Recombinant | E.coli. Applications: Bacterial (e. coli) nad(p)h-dependent fmn-oxidoreductase is a recombinant protein of ca. 26kda overexpressed in e.coli. the sequence of cloned fre (swissprot accession number p0aen1) was confirmed by dna sequencing (100% identity). Group: Enzymes. Synonyms: NAD(P)H:flavin oxidoreduct. Enzyme Commission Number: EC 1.5.1.29. Mole weight: 26kDa. Activity: >2U/mg. Appearance: Coupling of bacterial luciferase to FMN-NAD(P)H oxidoreductase has been used to provide ultrasensitive analytical tools for thequantification of NADH and the substrates of NADH-, NADPH- dependent enzymes (e.g. glucose, lactate, malate, ethanol, sorbitol,oxaloacetate). Although FMN-reductase often present in luciferase enzyme preparations may be sufficient for producing light in the presence of NAD(P)H, highly purified and characterized Fre enzyme can offer some advantages such as an increased sensitivity,better control of the signal intensity and duration, and saving of the luciferase enzyme. Species: FMN Reductase. NAD(P)H:flavin oxidoreductase; NAD(P)H:flavin mono-nucleotide oxidoreductase; NAD(P)H(2):FMN oxidoreductase; NAD(P)H-FMN reductase; NAD(P)H-dependent FMN reductase; NAD(P)H:FMN oxidoreductase; riboflavin mononucleotide reductase; flavin mononucleotide reductase; EC 1.5.1.29. Pack: stable lyophilized form. Cat No: NATE-1744. | |
| Fok I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 2-fold overdigestion with enzyme more than 95% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 100U; 500U. GGATG(N)9↑ CCTAC(N)13&darr. Activity: 1000-2000u.a./ml. Appearance: 10 X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned Fok I gene from Flavobacterium okeanokoites. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1112RE. | |
| Fpg Protein from Escherichia coli, Recombinant | Fpg protein, a key enzyme in the DNA base excision repair pathway (BER), catalyses the excision of a broad spectrum of modified purines such as formamidopyrimidine (Fapy) and 8-oxoguanine (8-oxo-G). Fpg possess both DNA glycosylase activity that removes the mutated base and AP-lyase activity that releases ribose, leaving both 5'-and 3'-phosphorylated ends in the DNA. Several analytical methods based on Fpg protein activity in vitro were developed for detection and quantitation of oxidative damage to DNA mainly for FapyA, FapyG and 8-oxo-G. The fpg gene was cloned by Boiteux, et al. Fpg protein possess a zinc finger motif at its C-terminus (one zinc atom per molecule). ... Protein. Mole weight: mol wt 30.2 kDa (269 amino acids, predicted from the nucleotide sequence). Activity: >20 ,000 units/mg protein. Storage: -20°C. Form: buffered aqueous glycerol solution; Solution in 50% glycerol containing 50 mM potassium HEPES, pH 7.5, 1 mM DTT, 1 mM EDTA, and 200 mM NaCl. Source: E. coli. Species: Escherichia coli. Fapy-DNA glycosylase; deoxyribonucleate glycosidase; 2,6-diamino-4-hydroxy-5N-formamidopyrimidine-DNA glycosylase; 2,6-diamino-4-hydroxy-5 (N-methyl)formamidopyrimidine-DNA glycosylase; formamidopyrimidine-DNA glycosylase; DNA-formamidopyrimidine glycosidase; Fpg protein; DNA-formamidopyrimidine glycosylase; EC 3.2.2.23; 78783-53-6; | |
| Fsp4H I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 5-fold overdigestion with enzyme about 5% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 200U; 1000U. GC↑NGC CGN↓CG. Activity: 3000-5000u.a./ml. Appearance: 10 X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned Fsp4H I gene from Flavobacterium species 4H. Pack: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 200ug/ml BSA; 1mM DTT; 50% glycerol. Cat No: ET-1114RE. | |
| Hae III | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 20-fold overdigestion with enzyme more than 90% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 2000U; 10000U. GG↑CC CC↓GG. Activity: 10000; 50000u.a./ml. Appearance: 10 X SE-buffer G. Storage: -20°C. Form: Liquid. Source: An E.coli strain, that carries the cloned Hae III gene from Haemophilus aegyptius. Pack: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1116RE. | |
| Hga I | One unit of the enzyme is the amount required to hydrolyze 1 μg of DNA pBR322 in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 3-fold overdigestion with enzyme more than 90% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 50U; 250U. GACGC(N)5↑ CTGCG(N)10&darr. Activity: 1000u.a./ml. Appearance: 10 X SE-buffer B. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned HgaI gene from Haemophilus gallinarum. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1117RE. | |
| Hind II | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 10-fold overdigestion with enzyme 60% of the dna fragments can be ligated and recut. in the presence of 10%peg ligation is better. Group: Restriction Enzymes. Purity: 1000U; 5000U. GTY↑RAC CAR↓YTG. Activity: 10000u.a./ml. Appearance: 10 X SE-buffer G, BSA. Storage: -20°C. Form: Liquid. Source: An E.coli strain, that carries the cloned gene HindII from Haemophilus influenzae. Pack: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1118RE. | |
| Hind III | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 50-fold overdigestion with enzyme more than 95% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 5000U; 25000U. A↑AGCTT TTCGA↓A. Activity: 20000; 50000u.a./ml. Appearance: 10 X SE-buffer W, BSA. Storage: -20°C. Form: Liquid. Source: An E.coli strain, that carries the cloned gene Hind III from Haemophilus influenzae Rd. Pack: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1119RE. | |
| Hinf I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 20-fold overdigestion with enzyme approximately 90% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 2000U; 10000U. G↑ANTC CTNA↓G. Activity: 20000u.a./ml. Appearance: 10 X SE-buffer O. Storage: -20°C. Form: Liquid. Source: An E.coli strain, that carries the cloned gene HinfI from Haemophilus influenzae. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1120RE. | |
| Hpa I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 5-fold overdigestion with enzyme about 60% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 500 U; 2500U. GTT↑AAC CAA↓TTG. Activity: 5000u.a./ml. Appearance: 10 X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: An E.coli strain, that carries the cloned gene Hpa I from Haemophilus parainfluenzae. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1121RE. | |
| Hpa II | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 10-fold overdigestion with enzyme more than 95% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 500 U; 2500U. C↑CGG GGC↓C. Activity: 10000u.a./ml. Appearance: 10 X SE-buffer B. Storage: -20°C. Form: Liquid. Source: An E.coli strain, that carries the cloned gene Hpa II from Haemophilus parainfluenzae. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 100 μg/ml BSA; 50% glycerol. Cat No: ET-1122RE. | |
| HpySE526 I | One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 5-fold overdigestion with enzyme about 95% of the dna fragments can be ligated with t4 dna ligase and recut. Group: Restriction Enzymes. Purity: 200U; 1000U. A↑CGT TGC↓A. Activity: 5000u.a./ml. Appearance: 10 X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: An E.coli strain, that carries the cloned HpySE 526I gene from Helicobacter pylori SE526. Pack: 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol. Cat No: ET-1123RE. | |
| HspA I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 20-fold overdigestion with enzyme more than 90% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 1000U; 5000U. G↑CGC CGC↓G. Activity: 20000u.a./ml. Appearance: 10 X SE-buffer Y. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned HspA I gene from Haemophilus species A1. Pack: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1124RE. | |
| Kpn I | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Applications: After 20-fold overdigestion with enzyme more than 90% of the dna fragments can be ligated and recut. Group: Restriction Enzymes. Purity: 2000U; 10000U. GGTAC↑C C↓CATGG. Activity: 20000u.a./ml. Appearance: 10 X SE-buffer B, BSA. Storage: -20°C. Form: Liquid. Source: An E.coli strain that carries the cloned Kpn I gene from Klebsiella pneumonia. Pack: 10 mM Tris-HCl(pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Cat No: ET-1125RE. | |
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