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Enterobactin is a siderophore produced by Gram-negative bacteria and has an extremely high affinity for iron. During the process of Salmonella typhimurium infecting macrophages, Enterobactin can not only help bacteria uptake iron but also reduce the antibacterial activity of macrophages. In addition, Enterobactin is also involved in the oxidative stress response of Escherichia coli. Enterobactin can be hydrolyzed by Fes in the cell and exert antioxidant effects by scavenging free radicals [1] [2]. Uses: Scientific research. Group: Natural products. CAS No. 28384-96-5. Pack Sizes: 10 mM * 1 mL; 1 mg; 5 mg; 10 mg. Product ID: HY-128525.
Enterobactin
It is produced by the strain of Escherichia coli, Salmonella typhi LT2. Enterobactin is a phenolic iron separation reagent and can be used as a growth accelerator. Synonyms: Enterochelin; tri-(2,3-dihydroxy-N-benzoyl-L-serine)-ester; tri-(N-(2,3-dihydroxybenzoyl)-L-serine)-ester; Enterochellin; N,N',N''-[(3S,7S,11S)-2,6,10-trioxo-1,5,9-trioxacyclododecane-3,7,11-triyl]tris(2,3-dihydroxybenzamide); Benzamide, N,N',N''-(2,6,10-trioxo-1,5,9-trioxacyclododecane-3,7,11-triyl)tris[2,3-dihydroxy-, [3S-(3R*,7R*,11R*)]-. CAS No. 28384-96-5. Molecular formula: C30H27N3O15. Mole weight: 669.55.
enterobactin synthase
This enzyme complex catalyses the conversion of three molecules each of 2,3-dihydroxybenzoate and L-serine to form the siderophore enterobactin. In Escherichia coli the complex is formed by EntB (an aryl carrier protein that has to be activated by 4'-phosphopantetheine), EntD (a phosphopantetheinyl transferase that activates EntB), EntE (catalyses the ATP-dependent condensation of 2,3-dihydroxybenzoate and holo-EntB to form the covalently arylated form of EntB), and EntF (a four domain protein that catalyses the activation of L-serine by ATP, the condensation of the activated L-serine with the activated 2,3-dihydroxybenzoate, and the trimerization of three such moieties to a single enterobactin molecule). Group: Enzymes. Synonyms: N-(2,3-dihydroxybenzoyl)-serine synthetase; 2,3-dihydroxybenzoylserine synthetase; 2,3-dihydroxybenzoate-serine ligase. Enzyme Commission Number: EC 6.3.2.14. CAS No. 37318-63-1. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-5735; enterobactin synthase; EC 6.3.2.14; 37318-63-1; N-(2,3-dihydroxybenzoyl)-serine synthetase; 2,3-dihydroxybenzoylserine synthetase; 2,3-dihydroxybenzoate-serine ligase. Cat No: EXWM-5735.
apo-Enterobactin
apo-Enterobactin is a derivative of enterobactin (enterochelin). Synonyms: Enterobactin, apo-. Grades: >95% by HPLC. CAS No. 30414-16-5. Molecular formula: C30H29N3O16. Mole weight: 687.6.
ferric-chelate reductase (NADPH)
Contains FAD. The enzyme, which is widespread among bacteria, catalyses the reduction of ferric iron bound to a variety of iron chelators (siderophores), including ferric triscatecholates and ferric dicitrate, resulting in the release of ferrous iron. The enzyme from the bacterium Escherichia coli has the highest efficiency with the hydrolysed ferric enterobactin complex ferric N-(2,3-dihydroxybenzoyl)-L-serine. cf. EC 1.16.1.7, ferric-chelate reductase (NADH) and EC 1.16.1.10, ferric-chelate reductase [NAD(P)H]. Group: Enzymes. Synonyms: ferric chelate reductase (ambiguous); iron chelate reductase (ambiguous); NADPH:Fe3+-EDTA reductase; NADPH-dependent ferric reductase; yqjH (gene name); Fe(II):NADP+ oxidoreductase. Enzyme Commission Number: EC 1.16.1.9. CAS No. 120720-17-4. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-1074; ferric-chelate reductase (NADPH); EC 1.16.1.9; 120720-17-4; ferric chelate reductase (ambiguous); iron chelate reductase (ambiguous); NADPH:Fe3+-EDTA reductase; NADPH-dependent ferric reductase; yqjH (gene name); Fe(II):NADP+ oxidoreductase. Cat No: EXWM-1074.
iron-chelate-transporting ATPase
ABC-type (ATP-binding cassette-type) ATPase, characterized by the presence of two similar ATP-binding domains. Does not undergo phosphorylation during the transport process. A bacterial enzyme that imports Fe-enterobactin, Fe-dicitrate, Fe-hydroxamate and other siderophores. Group: Enzymes. Enzyme Commission Number: EC 3.6.3.34. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-4672; iron-chelate-transporting ATPase; EC 3.6.3.34. Cat No: EXWM-4672.
isochorismatase
The enzyme is involved in the biosynthesis of several siderophores, such as 2,3-dihydroxybenzoylglycine, enterobactin, bacillibactin, and vibriobactin. Group: Enzymes. Synonyms: 2,3-dihydro-2,3-dihydroxybenzoate synthase; 2,3-dihydroxy-2,3-dihydrobenzoate synthase; 2,3-dihydroxy-2,3-dihydrobenzoic synthase. Enzyme Commission Number: EC 3.3.2.1. CAS No. 37288-64-5. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-3994; isochorismatase; EC 3.3.2.1; 37288-64-5; 2,3-dihydro-2,3-dihydroxybenzoate synthase; 2,3-dihydroxy-2,3-dihydrobenzoate synthase; 2,3-dihydroxy-2,3-dihydrobenzoic synthase. Cat No: EXWM-3994.
N,N'-bis(2,3-Dihydroxybenzoyl)-O-L-seryl-L-serine
N,N'-bis(2,3-Dihydroxybenzoyl)-O-L-seryl-L-serine is a microbial metabolite and degradation product of the bacterial siderophore enterobactin originally isolated from Streptomyces. It inhibits invasion of 26-L5 murine colon cancer cells in a Matrigel™ assay with an IC50 value of 2.7 μM. Synonyms: N-(2,3-dihydroxybenzoyl)-O-[N-(2,3-dihydroxybenzoyl)-L-seryl]-L-serine. Grades: >95% by HPLC. CAS No. 30414-15-4. Molecular formula: C20H20N2O11. Mole weight: 464.38.
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