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| Product | Description | |
|---|---|---|
| Nuclease-free Water | Nuclease-free Water. Ddh2o is purified by reverse osmosis method. it is suitable for most molecular and cell biology applications. Group: Cloning Enzymes. Purity: 25ml. Storage: Store at Room Temperature. Cat No: CE-3009. |  |
| Nuclease P1 | Nuclease P1 is a single-stranded specific endonuclease, it hydrolyzes nucleic acids into 5'-mononucleotides and cleaves the single-stranded region of a double-stranded nucleic acid. Nuclease P1 is one of the most well-known single stranded specific nucleases in the field of molecular biology, it is widely used in the pharmaceutical and food industries [1]. Nuclease P1 can be obtained by fermentation of Penicillium citrinum: through extraction process, ultrafiltration concentration, drying and purification, etc. Uses: Scientific research. Group: Signaling pathways. CAS No. 54576-84-0. Pack Sizes: 250 U. Product ID: HY-P2963. |  |
| Nuclease, restrictionendodeoxyribo-, BsmI | Heterocyclic Organic Compound. Alternative Names: BSM I;BSM I RESTRICTION ENZYME;RESTRICTION ENDONUCLEASES BSM I;restriction endonuclease bsm I*from bacillus;bsm i from bacillus stearothermophilus nub 36;RESTRICTION ENDONUCLEASE BSM I. CAS No. 122007-72-1. Catalog: ACM122007721. |  |
| Nuclease, restrictionendodeoxyribo-, FspI | Heterocyclic Organic Compound. Alternative Names: RESTRICTION ENDONUCLEASES NCO I;NCO I RESTRICTION ENZYME;NCO I;FSP I;restriction endonuclease nco I from*nocardia cora;RESTRICTION ENDONUCLEASE NCO I FROM*NOCA RDIA CORALL;nco i from nocardia corallina;RESTRICTION ENDONUCLEASE FSP I. CAS No. 107824-63-5. Catalog: ACM107824635. | |
| Nuclease, restrictionendodeoxyribo-, NotI | Heterocyclic Organic Compound. Alternative Names: NOT I;NOT I RESTRICTION ENZYME;RESTRICTION ENDONUCLEASES NOT I;restriction endonuclease not I from*nocardia otid;RESTRICTION ENDONUCLEASE NOT I FROM*NOCA RDIA OTIDIS;NOT I 5'.GC GGCCGC.3' ISOLATED FROM NOCARDIA OTITDIS-CAVIARUM;not i from nocardia ot. CAS No. 103780-20-7. Molecular formula: C13H18O5S2. Mole weight: 318.40902;g/mol. Purity: 0.96. IUPACName: [(2R,3S,4R)-4-acetyl-4-(1,3-dithian-2-yl)-3-methyl-5-oxooxolan-2-yl]acetate. Canonical SMILES: CC1C (OC (=O)C1 (C2SCCCS2)C (=O)C)OC (=O)C. Catalog: ACM103780207. | |
| Nuclease, restrictionendodeoxyribo-, SpeI | Heterocyclic Organic Compound. Alternative Names: RESTRICTION ENDONUCLEASES SPE I;SPE I;SPE I RESTRICTION ENZYME;RESTRICTION ENDONUCLEASE SPE I FROM*SPHA EROTILUS SP; spei5'. actagt. 3'isolatedfromsphaerotilusspecies; spe i from sphaerotilus sp.;RESTRICTION ENDONUCLEASE SPE I. CAS No. 115926-60-8. Molecular formula: ClH. Mole weight: 36.46094;g/mol. Purity: 0.96. IUPACName: chlorane. Canonical SMILES: Cl. ECNumber: 231-595-7. Catalog: ACM115926608. | |
| Nuclease S1, Aspergillus oryzae | Nuclease S1, Aspergillus oryzae is a specific endonuclease that degrades single stranded DNA (ssDNA) and RNA. Nuclease S1 can also remove protruding single-strand ends from double-stranded DNA [1] [2]. Uses: Scientific research. Group: Signaling pathways. CAS No. 37288-25-8. Pack Sizes: 10 KU. Product ID: HY-P2773. | |
| Nuclease, Serratia marcescens | Serratia marcescens nuclease (EC 3.1.30.2) is a nonspecific nuclease. Serratia marcescens nuclease has broad utility due to its potent digestive activity toward both DNA and RNA [1] [2]. Uses: Scientific research. Group: Signaling pathways. Alternative Names: Serratia marcescens nuclease. CAS No. 9025-65-4. Pack Sizes: 5 KU; 10 KU; 25 KU. Product ID: HY-131160. | |
| Aspergillus nuclease S1 | Aspergillus nuclease S1 is an endonuclease enzyme derived from Aspergillus oryzae that splits single-stranded DNA (ssDNA) and RNA into oligo- or mononucleotides. Group: Enzymes. Synonyms: endonuclease S1 (Aspergillus); single-stranded-nucleate endonuclease; deoxyribonuclease S1; deoxyribonuclease S1; nuclease S1; Neurospora crassa single-strand specific endonuclease; S1 nuclease; single-strand endodeoxyribonuclease; single-stranded DNA specific endonuclease; single-strand-specific endodeoxyribonuclease; single strand-specific DNase; Aspergillus oryzae S1 nuclease. Enzyme Commission Number: EC 3.1.30.1. CAS No. 37288-25-8. Nuclease. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-3707; Aspergillus nuclease S1; EC 3.1.30.1; 37288-25-8; endonuclease S1 (Aspergillus); single-stranded-nucleate endonuclease; deoxyribonuclease S1; deoxyribonuclease S1; nuclease S1; Neurospora crassa single-strand specific endonuclease; S1 nuclease; single-strand endodeoxyribonuclease; single-stranded DNA specific endonuclease; single-strand-specific endodeoxyribonuclease; single strand-specific DNase; Aspergillus oryzae S1 nuclease. Cat No: EXWM-3707. | |
| Cas9-C-NLS Nuclease | Cas9-C-NLS Nuclease. Cas9-c-nls nuclease is the recombinant streptococcus pyogenes cas9 (wt) protein with a c-terminal nucleic localization signal (nls). it can be used for genome editing by inducing site-specific dna double stranded breaks. cas9 protein forms a very stable ribonucleoprotein (rnp) complex with the guide rna (grna) component of the crispr/cas9 system, which can localize to the nucleus immediately once entering the cell with the guide of the nls. compared with the mrna or plasmid systems, transcription and translation processes are not required. this dna-free system avoids the risk of inserting foreign dna into the genome, which can be quite useful for gene editing-based disease therapy. our highly pure and active cas9 nuclease meets all of the researchers requirements (e.g. in vitro cleavage assay, rnp complex transfection, micro injection). Group: Cloning Enzymes. Purity: 50μg; 100μg. Storage: Store at -20 ?. Source: E.coli. Pack: 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol PH 7.4 at 25°C. Cat No: CE-3502. | |
| Cas9-N-NLS Nuclease | Cas9-N-NLS Nuclease. Cas9-n-nls nuclease is the recombinant streptococcus pyogenes cas9 (wt) protein with a n-terminal nucleic localization signal (nls) that can be used for genome editing by inducing site-specific dna double stranded breaks. cas9 protein forms a very stable ribonucleoprotein (rnp) complex with the guide rna (grna) component of the crispr/cas9 system, which can localize to the nucleus immediately once entering the cell with the guide of the nls. compared with the mrna or plasmid systems, transcription and translation processes are not required. this dna-free system avoids the risk of inserting foreign dna into the genome, which can be quite useful for gene editing-based disease therapy. our highly pure and active cas9 nuclease meets all of the researcher´s requirements (e.g. in vitro cleavage assay, rnp complex transfection, micro injection). Group: Cloning Enzymes. Purity: 50μg; 100μg. Storage: Store all components at -20 ?. Source: E.coli. Cat No: CE-3503. | |
| Cas9 Nuclease | Cas9 Nuclease. Recombinant streptococcus pyogenes cas9 (wt) nuclease is purified from e. coli.it can be used for genome editing by inducing site-specific double stranded breaks in double stranded dna. cas9 protein forms a very stable ribonucleoprotein (rnp) complex with the guide rna (grna) component of the crispr/cas9 system. the rnp complex recognizes the target site by matching grna with the genomic dna sequence and leads to dna breaks within 3 bases from the ngg pam (protospacer adjacent motif). with gencrispr cas9 nuclease, customers can screen highly efficient grna using in vitro dna cleavage assays. it could be a powerful tool for gene editing. Group: Cloning Enzymes. Purity: 10μg; 50μg. Storage: Store at -20 ?. Source: E.coli. Cat No: CE-3501. | |
| Deoxyribonuclease B, Recombinant | Deoxyribonuclease B (DNAse B) is one of several extracellular enzymes produced by group A beta-hemolytic streptococci. Since DNAse B is produced extensively by group A serotypes and is not produced in significant amounts by other serological groups (C and G), anti-DNAse B is a reliable streptococcal antibody test for both skin and throat infections. Applications: Used in the formulation of anti-dnase b assays which are used in the diagnosis of group a streptococcal based illnesses. Group: Enzymes. Synonyms: DNASE1; deoxyribonuclease I; deoxyribonuclease-1; DNase I; 9003-98-9; EC 3.1.21.1; pancreatic DNase; DNase; thymonuclease, dornase; dornava; dornavac; pancreatic deoxyribonuclease; pancreatic dornase; deoxyribonuclease (pancreatic); pancreatic DNas. Enzyme Commission Number: EC 3.1.21.1. Purity: > 97%. Mole weight: 21 - 31kD. Form: Frozen liquid. Source: Escherichia coli. DNASE1; deoxyribonuclease I; deoxyribonuclease-1; DNase I; 9003-98-9; EC 3.1.21.1; pancreatic DNase; DNase; thymonuclease, dornase; dornava; dornavac; pancreatic deoxyribonuclease; pancreatic dornase; deoxyribonuclease (pancreatic); pancreatic DNase; DNAase; deoxyribonucleic phosphatase; alkaline deoxyribonuclease; alkaline DNase; endodeoxyribonuclease I; DNA depolymerase; Escherichia coli endonuclease I; deoxyribonuclease A; DNA endonuclease; DNA nuclease. Cat No: NATE-1153. | |
| deoxyribonuclease I | Preference for double-stranded DNA. Group: Enzymes. Synonyms: pancreatic DNase; DNase; thymonuclease, dornase; dornava; dornavac; pancreatic deoxyribonuclease; pancreatic dornase; deoxyribonuclease (pancreatic); pancreatic DNase; DNAase; deoxyribonucleic phosphatase; DNase I; alkaline deoxyribonuclease; alkaline DNase; endodeoxyribonuclease I; DNA depolymerase; Escherichia coli endonuclease I; deoxyribonuclease A; DNA endonuclease; DNA nuclease. Enzyme Commission Number: EC 3.1.21.1. CAS No. 9003-98-9. DNASE1. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-3571; deoxyribonuclease I; EC 3.1.21.1; 9003-98-9; pancreatic DNase; DNase; thymonuclease, dornase; dornava; dornavac; pancreatic deoxyribonuclease; pancreatic dornase; deoxyribonuclease (pancreatic); pancreatic DNase; DNAase; deoxyribonucleic phosphatase; DNase I; alkaline deoxyribonuclease; alkaline DNase; endodeoxyribonuclease I; DNA depolymerase; Escherichia coli endonuclease I; deoxyribonuclease A; DNA endonuclease; DNA nuclease. Cat No: EXWM-3571. | |
| Deoxyribonuclease I from Bovine pastoris, Recombinant | Deoxyribonuclease I (usually called DNase I), is an endonuclease coded by the human gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. In addition to its role as a waste-management endonuclease, it has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis. Recombinant bovine pancreatic deoxyribonuclease 1 produced in pichia pastoris. chromatogr....1.21.1. CAS No. 9003-98-9. Purity: Chromatographically purified. DNASE1. Activity: > 5000 units per mg protein. Storage: Store at 2-8°C. Form: lyophilized powder. Source: Pichia pastoris. Species: Bovine pancreatic. DNASE1; deoxyribonuclease I; deoxyribonuclease-1; DNase I; 9003-98-9; EC 3.1.21.1; pancreatic DNase; DNase; thymonuclease, dornase; dornava; dornavac; pancreatic deoxyribonuclease; pancreatic dornase; deoxyribonuclease (pancreatic); pancreatic DNase; DNAase; deoxyribonucleic phosphatase; alkaline deoxyribonuclease; alkaline DNase; endodeoxyribonuclease I; DNA depolymerase; Escherichia coli endonuclease I; deoxyribonuclease A; DNA endonuclease; DNA nucle | |
| eSpCas9-N-NLS Nuclease | eSpCas9-N-NLS Nuclease. Espcas9 nuclease is a mutant form of cas9 nuclease. compared with the wild type cas9 nuclease, espcas9 reduces off-target effects by over 10-fold, while maintaining robust on-target genome editing efficiency. genscript has developed a espcas9-n-nls nuclease which contains a nuclear localization sequence (nls) on the n-terminus of the protein to meet all the researchers requirements (e.g. in vitro cleavage assay, rnp complex transfection, and micro injection). Group: Cloning Enzymes. Purity: 100μg; 500μg. Storage: Store at -20 ?. Source: E.coli. Pack: 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.4, at 25°C. Cat No: CE-3507. | |
| Micrococcal nuclease | Micrococcal nuclease is a biochemical reagent that can be used as a biological material or organic compound for life science related research. Uses: Scientific research. Group: Signaling pathways. CAS No. 9013-53-0. Pack Sizes: 5 KU. Product ID: HY-P2754. | |
| Micrococcal Nuclease | Micrococcal Nuclease. Micrococcal nuclease is obtained by expression of a recombinant micrococcal nuclease gene in escherichia coli, molecular weight is 16.8 kda. it is a non-specific exo or endonuclease that consumes double-stranded, single-stranded, circularized, and linear nucleic acids, and has a better digestion of single strands, and it tend to digest ats or the aus region of the dna or rna, producing a single nucleotide and a dinucleotide with 3' phosphoric acid.; high activity.; high protein purity, sds-page purity >95%.; good stability. Group: DNA Modifying Enzymes. Purity: 100KU; 200KU. Storage: Store at -20°C. Cat No: ME-4002. | |
| Mung Bean Nuclease, Reaction Buffer (10x) | Mung Bean Nuclease, Reaction Buffer (10x). Group: Molecular Biology. Grades: Molecular Biology Grade. Pack Sizes: 1ml. US Biological Life Sciences. |  Worldwide |
| Native Aspergillus oryzae Nuclease S1 | Nuclease S1 isolated from Aspergillus oryzae exhibits endo-and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5-phosphomononucleotide and 5-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form. The nuclease s1 enzyme from aspergillus oryzae has the ability to degrade single-stranded oligonucleotides composed of either deoxynucleotides or ribonucleotides. Applications: Nuclease s1 from aspergillus oryzae has been used in a study to assess a bi ochemical method for mapping mutational...e S1 nuclease; EC 3.1.30.1; 37288-25-8. Enzyme Commission Number: EC 3.1.30.1. CAS No. 37288-25-8. Nuclease. Storage: -20°C. Form: Solution containing 30 mM sodium acetate, 50 mM NaCl, 1 mM ZnCl2, 50% glycerol, 2 mg/ml protein. Source: Aspergillus oryzae. endonuclease S1 (Aspergillus); single-stranded-nucleate endonuclease; deoxyribonuclease S1; deoxyribonuclease S1; nuclease S1; Neurospora crassa single-strand specific endonuclease; S1 nuclease; single-strand endodeoxyribonuclease; single-stranded DNA specific endonuclease; single-strand-specific endodeoxyribonuclease; single strand-specific DNase; Aspergillus oryzae S1 nuclease; EC 3.1.30.1; 37288-25-8. Cat No: NATE-0492. | |
| Native Bovine Deoxyribonuclease I | Deoxyribonuclease I (usually called DNase I), is an endonuclease coded by the human gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. In addition to its role as a waste-management endonuclease, it has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis. Protein determined by biuret. Applications: Used for the removal of dna from protein samples. dnase...rom bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the dna association of minor groove-binding proteins reca and deoxyribonuclease i. Group: Enzymes. Synonyms: DNASE1; deoxyribonuclease I; deoxyribonuclease-1; DNase I; 9003-98-9; EC 3.1.21.1; pancreatic DNase; DNase; thymonuclease, dornase; dornava; dornavac; pancreatic deoxyribonuclease; pancreatic dornase; deoxyribonuclease (pancreatic); pancreatic DNase; DNAase; deoxyribonucleic phosphatase; alkaline deoxyribonuclease; alkaline DNase; endodeoxyribonuclease I; DNA depolymerase; Escherichia coli endonuclease I; deoxyribonuclease A; DNA end | |
| Native Bovine Deoxyribonuclease I RNase-free solution | Deoxyribonuclease I (usually called DNase I), is an endonuclease coded by the human gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. In addition to its role as a waste-management endonuclease, it has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis. Applications: Used in molecular biology applications for removing dna during rna purifica...zyme Commission Number: EC 3.1.21.1. CAS No. 9003-98-9. DNASE1. Mole weight: mol wt 29.1 kDa. Storage: -20°C. Form: buffered aqueous glycerol solution. Source: Bovine pancreas. Species: Bovine. DNASE1; deoxyribonuclease I; deoxyribonuclease-1; DNase I; 9003-98-9; EC 3.1.21.1; pancreatic DNase; DNase; thymonuclease, dornase; dornava; dornavac; pancreatic deoxyribonuclease; pancreatic dornase; deoxyribonuclease (pancreatic); pancreatic DNase; DNAase; deoxyribonucleic phosphatase; alkaline deoxyribonuclease; alkaline DNase; endodeoxyribonuclease I; DNA depolymerase; Escherichia coli endonuclease I; deoxyribonuclease A; DNA endonuclease; DNA nuclease. Cat No: NATE-0199. | |
| Native Penicillium citrinum Nuclease P1 | Nuclease P1 from Penicillium citrinum is a zinc-dependent endonuclease that exhibits increased activity in the presence of low concentrations of urea. Applications: Nuclease p1 from penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent. it has also been used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5? or 3? end group labeled rna. nuclease p1 is used to improve the sensitivity of a 32p-labeling method for the detection of dna adducts. the enzyme has an optimal temperature of approximately 70 oc, but for a long incubation, a temperature below 60 oc is more suitable. it is stable in the ph range of 5-8. Group: Enzymes. Synonyms: Endonuclease P1; EC 3.1.30.1; 54576-84-0; Nuclease P1; P1 nuclease. Enzyme Commission Number: EC 3.1.30.1. CAS No. 54576-84-0. Nuclease. Mole weight: 42-50 kDa. Activity: > 200 units/mg protein (E1%/280, 3?-5?-Phosphodiesterase). Storage: 2-8°C. Form: lyophilized powder. Source: Penicillium citrinum. Endonuclease P1; EC 3.1.30.1; 54576-84-0; Nuclease P1; P1 nuclease. Pack: vial of > 250 units (using RNA substrate). Cat No: NATE-0491. | |
| Native Staphylococcus aureus Nuclease micrococcal | Micrococcal Nuclease is an endo-exonuclease that preferentially digests single-stranded nucleic acids. The rate of cleavage is 30 times greater at the 5' side of A or T than at G or C and results in the production of mononucleotides and oligonucleotides with terminal 3'-phosphates. The enzyme is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved. Applications: Nuclease from staphyl oc occus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. it has also been used in a study to investigate the expression characteristic of two genes in s. aureus that encode two thermostable nucleases. Group: Enzymes. Synonyms: Micrococcal Nuclease; EC 3.1.31.1; spleen endonuclease; thermonuclease; nuclease T; micrococcal endonuclease; nuclease T'; staphylococcal nucle. Enzyme Commission Number: EC 3.1.31.1. CAS No. 9013-53-0. MNase. Activity: 100-300 units/mg protein. Storage: -20°C. Source: Staphylococcus aureus. Micrococcal Nuclease; EC 3.1.31.1; spleen endonuclease; thermonuclease; nuclease T; micrococcal endonuclease; nuclease T'; staphylococcal nuclease; spleen phosphodiesterase; Staphylococcus aureus nuclease; Staphylococcus aureus nuclease B; ribonucleate (deoxynucleate) 3'-nucleotidohydrolase; 9013-53-0; Endonuclease micrococcal; MNase. Cat No: NATE-0452. | |
| NLS-Cas9-EGFP Nuclease | NLS-Cas9-EGFP Nuclease. Nls-cas9-egfp is a fusion protein contains a nuclear localization sequence (nls) on its n terminal and egfp on the c terminal. the egfp can be used as a reporter for tracking or sorting transfected cells, which enables the possibility of enriching cell populations for desired genome edits via fluorescence activated cell sorting (facs). it significantly reduces the labor and cost associated with single cell cloning and genotyping in genome editing applications. Group: Cloning Enzymes. Purity: 100μg; 500μg. Storage: Store at -20 ?. Source: E.coli. Pack: 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA,1 mM DTT, 50% Glycerol PH7.4, at 25°C. Cat No: CE-3506. | |
| NLS-Cas9-NLS Nuclease | NLS-Cas9-NLS Nuclease. Nls-cas9-nls is produced by expression in an e. coli strain carrying a plasmid encoding the cas9 gene from streptococcus pyogenes with a double-ends nuclear localization signal (nls). it can be used for genome editing by inducing site-specific dna double stranded breaks. cas9 protein forms a very stable ribonucleoprotein (rnp) complex with the guide rna (grna) component of the crispr/cas9 system, which can localize to the nucleus immediately once entering the cell with the guide of the nls. compared with the mrna or plasmid systems, transcription and translation processes are not required. this dna-free system avoids the risk of inserting foreign dna into the genome, which can be quite useful for gene editing-based disease therapy. our highly pure and active cas9 nuclease meets all of the researcher´s requirements (e.g. in vitro cleavage assay, rnp complex transfection, micro injection). Group: Cloning Enzymes. Purity: 50μg; 100μg. Storage: Store at -20 ?. Source: E.coli. Pack: 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.4 at 25?. Cat No: CE-3504. | |
| ribonuclease H | Acts on RNA-DNA hybrids. Group: Enzymes. Synonyms: endoribonuclease H (calf thymus); RNase H; RNA*DNA hybrid ribonucleotidohydrolase; hybrid ribonuclease; hybridase; hybridase (ribonuclease H); ribonuclease H; hybrid nuclease; calf thymus ribonuclease H. Enzyme Commission Number: EC 3.1.26.4. CAS No. 9050-76-4. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-3592; ribonuclease H; EC 3.1.26.4; 9050-76-4; endoribonuclease H (calf thymus); RNase H; RNA*DNA hybrid ribonucleotidohydrolase; hybrid ribonuclease; hybridase; hybridase (ribonuclease H); ribonuclease H; hybrid nuclease; calf thymus ribonuclease H. Cat No: EXWM-3592. | |
| ribonuclease M5 | Converts the 5S-rRNA precursor from Bacillus subtilis into 5S-rRNA, with 5'-phosphate and 3'-hydroxy groups. Group: Enzymes. Synonyms: RNase M5; 5S ribosomal maturation nuclease; 5S ribosomal RNA maturation endonuclease. Enzyme Commission Number: EC 3.1.26.8. CAS No. 62253-00-3. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-3596; ribonuclease M5; EC 3.1.26.8; 62253-00-3; RNase M5; 5S ribosomal maturation nuclease; 5S ribosomal RNA maturation endonuclease. Cat No: EXWM-3596. | |
| Serratia marcescens nuclease | Hydrolyses double- or single-stranded substrate. Group: Enzymes. Synonyms: endonuclease (Serratia marcescens); barley nuclease; plant nuclease I; nucleate endonuclease. Enzyme Commission Number: EC 3.1.30.2. CAS No. 9025-65-4. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-3708; Serratia marcescens nuclease; EC 3.1.30.2; 9025-65-4; endonuclease (Serratia marcescens); barley nuclease; plant nuclease I; nucleate endonuclease. Cat No: EXWM-3708. | |
| Water, Sterile, Nuclease & Endotoxin Free | United States Biological produces ultrapure water suitable in all molecular biology procedures and available at very reasonable pricing. Produced under cGMP. Group: Biochemicals. Grades: Molecular Biology Grade. CAS No. 7732-18-5. Pack Sizes: 50ml, 100ml, 250ml, 500ml, 1L. US Biological Life Sciences. | Worldwide |
| Water, Sterile, Nuclease Free | United States Biological produces ultrapure water suitable in all molecular biology procedures and available at very reasonable pricing. Produced under cGMP. Group: Biochemicals. Grades: Molecular Biology Grade. CAS No. 7732-18-5. Pack Sizes: 500ml, 1L, 3L. US Biological Life Sciences. | Worldwide |
| 1,10-Phenanthroline monohydrate, 99% | 1,10-Phenanthroline monohydrate, 99%. Uses: When complexed with copper, it possesses nuclease activity that has been used to study dna-protein interactions. Group: Ligands for functional metal complexes. Alternative Names: ZX-AT006983; SCHEMBL3790396; KSC269G7H; UNII-KSX215X00E; 1,10-phenanthroline-hydrate; TL8003406; 1,10-Phenanthroline hydrate; AX8021147; 1,10-Phenanthroline monohydrate, ACS reagent, 99%; TR-018323. CAS No. 5144-89-8. Product ID: 1,10-phenanthroline; hydrate. Molecular formula: 198.225g/mol. Mole weight: C12H10N2O. C1=CC2=C(C3=C(C=CC=N3)C=C2)N=C1.O. InChI=1S/C12H8N2. H2O/c1-3-9-5-6-10-4-2-8-14-12 (10)11 (9)13-7-1; /h1-8H; 1H2. PPQJCISYYXZCAE-UHFFFAOYSA-N. |  |
| 1,10-Phenanthroline monohydrate, 99% | When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions. Group: Heterocyclic organic compound. Alternative Names: ZX-AT006983; SCHEMBL3790396; KSC269G7H; UNII-KSX215X00E; 1,10-phenanthroline-hydrate; TL8003406; 1,10-Phenanthroline hydrate; AX8021147; 1,10-Phenanthroline monohydrate, ACS reagent, 99%; TR-018323. CAS No. 5144-89-8. Molecular formula: C12H10N2O. Mole weight: 198.225g/mol. IUPACName: 1,10-phenanthroline;hydrate. Canonical SMILES: C1=CC2=C(C3=C(C=CC=N3)C=C2)N=C1.O. Catalog: ACM5144898. | |
| 1,2,3,4,6-Penta-O-acetyl-5-thio-b-D-galactopyranoside | Penta-O-acetyl-5-thio-b-D-galactopyranoside, a chemical compound, may be employed as a substance to gauge enzyme function, with a particular preference for esterases, nucleases, and glycosidases. Additionally, the compound's effectiveness extends to protein purification. |  |
| 2'-Deoxy-2'-fluoroguanosine-5'-triphosphate tetralithium salt | 2'-Deoxy-2'-fluoroguanosine-5'-triphosphate tetralithium salt, a compound extensively utilized in the biomedical industry for nucleic acid research and diagnostics, stands as a vital reagent. Its significance lies in its role as a fundamental constituent in the synthesis of modified DNA strands, bolstered by the outstanding stability and nuclease resistance granted by its distinctive fluorine substitution. The manifold applications of this compound encompass drug discovery, genetic engineering, as well as investigations pertaining to antiviral agents. Synonyms: 2'-Deoxy-2'-fluoro-D-guanosine-5'-triphosphate. Grades: 95%. CAS No. 202186-97-8. Molecular formula: C10H15FN5O13P3·xLi. Mole weight: 525.17 (free acid). | |
| 2'-F-A-ANA-CE Phosphoramidite | 2'-F-A-ANA-CE Phosphoramidite is a reagent used in the biomedical industry to synthesize oligonucleotides, particularly those used in gene silencing and antisense therapy. Its modified nucleobase enhances binding specificity and stability against nuclease degradation. Synonyms: 5'-Dimethoxytrityl-N6-benzoyl-2'-deoxy-2'-fluoroarabinoadenosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5'-O-DMT-N6-Benzoyl-2'-fluoro-2'-arabinofuranosyl-2'-deoxyadenosine 3'-CE phosphoramidite. Molecular formula: C47H51FN7O7P. Mole weight: 875.93. | |
| 2'-F-Ac-C-ANA-CE Phosphoramidite | 2'-F-Ac-C-ANA-CE Phosphoramidite is a remarkably efficacious phosphoramidite reagent, extensively employed in intricate oligonucleotide synthesis endeavors. This paramount constituent functions as a fundamental unit in fabricating nucleic acids bearing ameliorated characteristics, specifically in the realm of antiviral functionality. The integration of 2'-F-Ac-C modification augments deftness against nuclease degradation and guarantees heightened stability, rendering it exceptionally congruous for the purpose of combatting viral afflictions. Synonyms: 5'-Dimethoxytrityl-N4-acetyl-2'-deoxy-2'-fluoroarabinocytidine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite. Molecular formula: C41H49FN5O8P. Mole weight: 789.83. | |
| 2'-F-dTTP | 2'-F-dTTP is a modified nucleoside triphosphate that increases the stability of nucleases. Synonyms: 2'-Fluoro-thymidine-5'-Triphosphate. Grades: ≥95% by AX-HPLC. Molecular formula: C10H16N2O14P3F. Mole weight: 500.16. | |
| 2'-O-Methyl-5-methyl-UTP | 2'-O-Methyl-5-methyl-UTP is a sugar-modified nucleoside triphosphate that increases target affinity and nuclease stability, while reducing immune response. Synonyms: 2'-O-Methyl-5-methyluridine-5'-Triphosphate. Grades: ≥90% by AX-HPLC. Molecular formula: C11H19N2O15P3. Mole weight: 512.19. | |
| 2'-O-tert-Butyldimethylsilyl-5'-O-DMT-N-dibutylformamide-isocytidine 3'-CE phosphoramidite | 2'-O-tert-Butyldimethylsilyl-5'-O-DMT-N-dibutylformamide-isocytidine 3'-CE phosphoramidite is a highly specialized and indispensable building block utilized in the intricate synthesis of RNA and DNA strands, careening through the impermeable terrain of nucleic acid research and development. It propels researchers towards the ultimate goal of introducing a phosphorothioate linkage at the prestigious 3'-end of oligonucleotide synthesis, effectively impeding nuclease degradation with magnificent fortitude. Behold, as this product facilitates the development of novel anti-cancer drugs, gene therapy, and RNA interference (RNAi) technologies with unparalleled excellence. Molecular formula: C54H79N6O8PSi. Mole weight: 999.30. | |
| 3'-BHQ-3 CPG | 3'-BHQ-3 CPG, a modified nucleotide, is a vital component of oligonucleotide synthesis for scientific research. The merging of black hole quencher (BHQ) and 3' cytosine (C) modification endows it with magnified quenching capacity for fluorescent probes and enhanced nuclease stability that is imperative in the domain of diagnostics and therapeutics for addressing viral, cancerous, and immune disorders. Synonyms: 3-Diethylamino-5-phenylphenazium-7-diazobenzene-4''-(N-ethyl-2-O-(4,4'-dimethoxytrityl))-N-ethyl-2-O-glycolate-CPG. Mole weight: 597.63. | |
| 3'-O-allyl-2'-deoxythymidine-5'-triphosphate trisodium salt | 3'-O-allyl-2'-deoxythymidine-5'-triphosphate trisodium salt: a substrate utilized in enzymatic reactions, PCR and sequencing, is ideal for improving nuclease resistance and duplex stability when synthesizing modified DNA oligos. Its multifaceted potential is truly remarkable. Synonyms: 3'-O-allyl-2'-dTTP·Na3. Grades: ≥97% by HPLC. Molecular formula: C13H18N2Na3O14P3. Mole weight: 588.18. | |
| 3'-O-DMT-N6-Benzoyl-Adenosine TNA 2'-CE phosphoramidite | 3'-O-DMT-N6-Benzoyl-Adenosine TNA 2'-CE phosphoramidite is a modified nucleotide used in the synthesis of Threose Nucleic Acid (TNA) molecules for biomedical research. TNA is a promising alternative to DNA and RNA for gene and drug delivery due to its chemical stability and resistance to nuclease degradation. This specific phosphoramidite derivative incorporates a benzoyl-adenosine moiety, making it ideal for designing TNA-based drugs for the treatment of viral infections, inflammatory diseases, and cancer. Synonyms: N-Benzoyl-9-[(2R)-3alpha-[(diisopropylamino)(2-cyanoethoxy)phosphinooxy]-4beta-(4,4'-dimethoxytrityloxy)tetrahydrofuran-2beta-yl]-9H-purine-6-amine; A-TNA Phosphoramidite. Grades: ≥97% by HPLC. Molecular formula: C46H50N7O7P. Mole weight: 843.92. | |
| 3'-Spacer C3 CPG 1000 | 3'-Spacer C3 CPG 1000, a widely utilized biomedicine industry product, is vital for synthesizing antisense oligonucleotides. With its ability to be solidly supported on the 3' end of oligonucleotide sequences, 3'-Spacer C3 CPG 1000 enhances hybridization properties whilst offering nuclease resistance. | |
| 5-Br-CTP | 5-Br-CTP is a base modified ribonucleoside triphosphate used in SELEX technology to provide thermal stability and nuclease resistance to aptamers. Synonyms: 5-Bromocytidine-5'-Triphosphate. Grades: ≥90% by AX-HPLC. Molecular formula: C9H15N3O14P3Br. Mole weight: 562.05. | |
| 5-Br-dU-CE Phosphoramidite | 5-Br-dU-CE Phosphoramidite, a chemical compound used in oligonucleotide synthesis, boasts a 5-bromo-2'-deoxyuridine (Br-dU) group that is linked to a cytosine base, thereby elevating base-pairing specificity and amplifying nuclease resistance. Its potential applications in the realm of biomedical research, encompassing cancer treatment, antiviral agents, and gene therapy, warrant close scrutiny. Synonyms: 5'-Dimethoxytrityl-5-bromo-2'-deoxyUridine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite. Molecular formula: C39H46BrN4O8P. Mole weight: 809.69. | |
| 5-Br-UTP | 5-Br-UTP is a base modified ribonucleoside triphosphate used in SELEX technology to provide thermal stability and nuclease resistance to aptamers. Synonyms: 5-Bromouridine-5'-Triphosphate. Grades: ≥90% by AX-HPLC. Molecular formula: C9H14N2O15P3Br. Mole weight: 563.0. | |
| 5'-O-DMT-N6-Acetyl-2'-deoxyadenosine 3'-CE phosphoramidite | 5'-O-DMT-N6-Acetyl-2'-deoxyadenosine 3'-CE phosphoramidite is a key component for the synthesis of modified oligonucleotides, which have applications in gene therapy and antiviral drug development. This phosphoramidite can be used to introduce an N6-acetyldeoxyadenosine modification to oligonucleotide strands, which has been shown to enhance their cellular uptake and improve their stability towards nucleases. It is also useful for generating oligonucleotides with 3'-CE modifications, which can improve their binding and selectivity towards complementary RNA targets. Synonyms: Adenosine N-acetyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-deoxy-, 3'-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]; N-Acetyl-5'-O-[bis (4-methoxyphenyl) (phenyl)methyl]-3'-O-[ (2-cyanoethoxy) (diisopropylamino)phosphino]-2'-deoxyadenosine. Grades: ≥98% by HPLC. CAS No. 1027734-01-5. Molecular formula: C42H50N7O7P. Mole weight: 795.86. | |
| 5'-O-DMT-thymidine 3'-thiophosphoramidite | 5'-O-DMT-thymidine 3'-thiophosphoramidite, a highly indispensable reagent employed in numerous biomedical applications, has the capability to confer augmented stability and safeguard the resultant sequences against degradation by nucleases. Its employment facilitates the precise introduction of 5'-O-DMT-thymidine into the oligonucleotide chain, thereby enabling focused therapeutic interventions for drug administration, gene suppression, and pathological condition detection. Synonyms: 5'-Dimethoxytrityl-2'-deoxythymidine 3'-[(B-thiobenzoylethyl)-(1-pyrrolidinyl)]-thiophosphoramidite; dT-Thiophosphoramidite. Grades: 90%. Molecular formula: C44H48N3O8PS2. Mole weight: 841.97. | |
| 7-Propargylamino-7-deaza-dGTP - ATTO-580Q | 7-Propargylamino-7-deaza-dGTP - ATTO-580Q is a fluorescent nucleotide analog used for labeling DNA during polymerase chain reaction (PCR) or sequencing. It can specifically label adenine bases and is useful for detecting mutations or identifying DNA-protein interactions. Its unique structure also makes it resistant to nucleases and exonucleases. Synonyms: 7-Deaza-7-propargylamino-2'-deoxyguanosine-5'-triphosphate, labeled with ATTO 580Q, Triethylammonium salt. Grades: ≥ 95% by HPLC. Molecular formula: C14H20N5O13P3- ATTO 580Q (free acid). Mole weight: 1235.25 (free acid). | |
| Aluminon | Aluminon (ATA; Ammonium aurintricarboxylate; Aurintricarboxylic acid ammonium) is a complexometric titration indicator and can also be used as a nuclease inhibitor affecting transfection. Aluminon is a kind of biological materials or organic compounds that are widely used in life science research [1]. Uses: Scientific research. Group: Biochemical assay reagents. Alternative Names: ATA; Ammonium aurintricarboxylate; Aurintricarboxylic acid ammonium. CAS No. 569-58-4. Pack Sizes: 5 g. Product ID: HY-124493. | |
| Brivudine | Brivudine, also known as bromovinyl-deoxyuridine, is a uridine derivative and nucleoside analog with pro-apoptotic and chemosensitizing properties. In vitro, bromovinyl-deoxyuridine (BVDU) has been shown to downregulate the multifunctional DNA repair enzyme APEX nuclease 1, resulting in the inhibition of DNA repair and the induction of apoptosis. In addition, this agent may inhibit the expression of STAT3 (signal transducer and activator of transcription 3), which may result in the downregulation of vascular endothelial growth factor (VEGF). BVDU has also been found to inhibit the upregulation of chemoresistance genes (Mdr1 and DHFR) during chemotherapy. Overall, the gene expression changes associated with BVDU treatment result in the decrease or prevention of chemoresistance. In addition, this agent has been shown to enhance the cytolytic activity of NK-92 natural killer cells towards a pancreatic cancer cell line in vitro. Synonyms: (E)-5-(2-Bromovinyl)-2'-deoxyuridine; (E)-5-(2-Bromovinyl)deoxyuridine; (E)-5-O-(2-bromoethenyl)-2'-deoxyuridine; 5-[(1E)-2-Bromoethenyl]-2'-deoxyuridine; 5-[(E)-2-Bromoethenyl]-2'-deoxyuridine; BVDU; Brivudin; Bromovinyldeoxyuridine; Helpin. Grades: ≥98% by HPLC. CAS No. 69304-47-8. Molecular formula: C11H13BrN2O5. Mole weight: 333.14. | |
| dG-Thiophosphoramidite | dG-Thiophosphoramidite, a premium reagent, is widely utilized in biomedical research. Its application lies in the synthesis of DNA oligonucleotides equipped with thiophosphate links, rendering them incredibly potent agents for combating diseases such as cancer and viral infections, courtesy of enhanced nuclease resistance. Researchers entrust this reagent for its dependable performance as it is easily accessible and has a sound reputation. Synonyms: 5'-Dimethoxytrityl-N-isobutyryl-2'-deoxyGuanosine, 3'-[(ß-thiobenzoylethyl)-(1-pyrrolidinyl)]-thiophosphoramidite. Molecular formula: C48H53N6O8PS2. Mole weight: 937.07. | |
| Diethylpyrocarbonate (DEPC) | DEPC is a strong nuclease inhibitor, utilizing a mechanism which modifies His and Tyr residues in proteins. Used as a RNase and DNase inhibitor for Molecular Biology solutions. DEPC is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen. Group: Biochemicals. Alternative Names: Diethyloxydiformate; Ethyloxyformic acid anhydride; Diethyl dicarbonate; DEP; DEPC; EC 216-542-8. Grades: Molecular Biology Grade. CAS No. 1609-47-8. Pack Sizes: 25g, 50g, 100g. US Biological Life Sciences. | Worldwide |
| Diethyl Pyrocarbonate (DEPC) 99+% (GC) | DEPC is a strong nuclease inhibitor, utilizing a mechanism which modifies His and Tyr residues in proteins. Used as a RNase and DNase inhibitor for Molecular Biology solutions. DEPC is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen. Group: Biochemicals. Alternative Names: Diethyloxydiformate; Ethyloxyformic acid anhydride; Diethyl dicarbonate; DEP; DEPC; EC 216-542-8. Grades: Molecular Biology Grade. CAS No. 1609-47-8. Pack Sizes: 5g, 25g, 100g, 250g. Molecular Formula: C6H10O5, Molecular Weight: 162.14. US Biological Life Sciences. | Worldwide |
| EDTA Disodium Salt, 0.25M Solution | Ultrapure solution for electrophoresis, manufactured with 99%+ EDTA and Molecular Biology Grade water. Sterile and nuclease free. Group: Biochemicals. Alternative Names: Edetate disodium salt dihydrate; Disodium ethylene diaminetetraacetate dihydrate; Edathamil; Ethylene diaminetetraacetic acid disodium salt dihydrate; EDTA-Na2; Sequestrene Na2. Grades: Molecular Biology Grade. CAS No. 6381-92-6. Pack Sizes: 100ml, 500ml, 1L, 2.5L, 4L. Molecular Formula: C10H14N2O8Na2 2H2O, Molecular Weight: 372.24. US Biological Life Sciences. | Worldwide |
| Guanidine thiocyanate | Guanidine thiocyanate is a strong protein denaturant and potent inhibitor of nucleases. Guanidinium thiocyanate is a nucleic acid protector in the extraction of DNA and RNA from cells. Guanidine thiocyanate is a common component of buffers used for nucleic acid extraction [1]. Uses: Scientific research. Group: Natural products. CAS No. 593-84-0. Pack Sizes: 10 mM * 1 mL; 25 g. Product ID: HY-D0841. | |
| Lucanthone | Lucanthone is orally available thioxanthone-based DNA intercalator and inhibitor of the DNA repair enzyme apurinic-apyrimidinic endonuclease 1 (APEX1 or APE1), with anti-schistosomal and potential antineoplastic activity. Lucanthone intercalates DNA and interferes with the activity of topoisomerases I and II during replication and transcription, thereby inhibiting the synthesis of macromolecules. In addition, this agent specifically inhibits the endonuclease activity of APE1, without affecting its redox activity, resulting in unrepaired DNA strand breaks which may induce apoptosis. Therefore, lucanthone may sensitize tumor cells to radiation and chemotherapy. Furthermore, lucanthone inhibits autophagy through the disruption of lysosomal function. The multifunctional nuclease APE1 is a key component for DNA repair; its expression is often correlated with tumor cell resistance to radio- and chemotherapy. Uses: Schistosomicides. Synonyms: Lucanthonum; 1-{[2-(Diethylamino)ethyl]amino}-4-methyl-9H-thioxanthen-9-one; CCRIS 1106. CAS No. 479-50-5. Molecular formula: C20H24N2OS. Mole weight: 340.48. | |
| Mutation Detection Kit | Mutation Detection Kit. Mutation detection kit provides a simple, reliable, and rapid method for the detection of site specific cleavage of genomic dna that is extracted from cells transfected with constructs expressing engineered nucleases such as transcription activator-like effector nucleases (talen), clustered regularly interspaced short palindromic repeats (crispr)/cas9, or zinc-finger nucleases (zfn). mutation detection kit includes high-fidelity dna polymerase for amplifying the target regions from cells, and t7 endonuclease i for recognizing and detecting the mismatches caused by gene editing tools. it provides an easy and reliable approach for estimating the efficiency of genome editing. components. high-fidelity dna polymerase. 5x pcr reaction buffer. t7 endonuclease i. 10x gencrispr t7 endonuclease i reaction buffer. control template dna. control primer mix. protease k. dntp. Group: Cloning Enzymes. Purity: 25 reactions/kit. Storage: Store at -20 ?. Source: E.coli. Pack: 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.4, at 25°C. Cat No: CE-3508. | |
| N6-Benzoyl-5'-O-DMT-2'-O-(2-methoxyethyl)adenosine 3'-CE phosphoramidite | N6-Benzoyl-5'-O-DMT-2'-O-(2-methoxyethyl)adenosine 3'-CE phosphoramidite is used to incorporate 2'-O-methoxyethyl-modified A into oligonucleotide phosphoramidites, which is useful for antisense RNA. The 2'-MOE backbone provides stronger duplex stability, significant nuclease resistance and relatively low toxicity. Synonyms: N6-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(2-methoxyethyl)adenosine-3'-O-[(2-cyanoethyl)(N,N-diisopropyl)]-phosphorramidite; N6-Bz-5'-O-DMTr-2'-O-(2-methoxyethyl)-adenosine-3'-CED-phosphoramidite; 2'-O-MOE-A(Bz)-3'-phosphoramidite; N6-Benzoyl-5'-O-DMT-2'-O-MOE-adenosine 3'-CE phosphoramidite; N-Benzoyl-5'-O-[bis (4-methoxyphenyl) (phenyl)methyl]-3'-O-[ (2-cyanoethoxy) (diisopropylamino)phosphino]-2'-O- (2-methoxyethyl)adenosine. Grades: ≥95%. CAS No. 251647-53-7. Molecular formula: C50H58N7O9P. Mole weight: 932.01. | |
| Native Tritirachium album limber Proteinase K | Proteinase K (PROK) is a serine protease with broad specificity towards aliphatic, aromatic and other hydrophobic amino acids. PROK has a molecular weight of 27,000 daltons and is Ca2+ dependent. It is not inactivated by metal ion chelating agents such as EDTA, sulfhydryl reagents, PCMB, TLCK, or TPCK. It also retains activity in 0.5% SDS. It can be inhibited by PMSF or DFP. Applications: Useful for the proteolytic inactivation of nucleases during the isolation of dna and rna. removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease a. reported useful for the isolation of hepatic, yeast, and mung bean mit ochondria determination of enzyme l o...oteinase; Tritirachium album proteinase K; endopeptidase K; 39450-01-6; protease K. Enzyme Commission Number: EC 3.4.21.64. CAS No. 39450-01-6. Purity: Purified to remove DNase and RNase. Proteinase K. Mole weight: 27 kDa. Activity: Type I, > 20 units per mg dry weight; Type II, > 400 u/ml. Storage: Powder: 2-8°C; Liquid: -20°C. Form: Type I, powder; Type II, Liquid in 20mg/ml in 10mM Tris-HCl, 1mM calcium acetate, pH 7.5 containing 50% glycerol. Source: Tritirachium album limber. Proteinase K; EC 3.4.21.64; Tritirachium alkaline proteinase; Tritirachium album serine proteinase; Tritirachium album proteinase K; endopeptidase K; 39450-01-6; protease K. Cat No: NATE-0637. | |
| Native Tritirachium album Proteinase K | Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments:pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. Applications: Proteinase k is useful for the proteolytic inactivation of nucleases during the isolation of dna and rna. it is used for the removal of endotoxins bo... used to facilitate the access of probes to rrna using fish techniques to detect pathogenic staphylococcus aureus. useful for the proteolytic inactivation of nucleases during the isolation of dna and rna. removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease a. reported useful for the isolation of hepatic, yeast, and mung bean mitochondria determination of enzyme localization on membranes treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. digestion of proteins from brain tissue samples for prions in transmissible spongiform encephalopathies (tse) research. Group: Enzymes. Synonyms: Tritirachiu | |
| NK314 | NK314 is a novel synthetic benzo[c]phenanthridine alkaloid that shows strong antitumor activity. It inhibited topoisomerase II activity and stabilized topoisomerase II-DNA cleavable complexes. The DNA breaks occurred within 1h after treatment with NK314 even without digestion of topoisomerase II by proteinase K, whereas etoposide required digestion of the enzyme protein in cleavable complex to detect DNA breaks. Pretreatment with topoisomerase II catalytic inhibitors, ICRF-193 and suramin, reduced both cleavable complex-mediated DNA breaks and proteinase K-independent DNA breaks, but protease inhibitors and nuclease inhibitors only decreased the latter. These results indicate that NK314 might affect topoisomerase II in the different manner from cleavable complex formation and activate intracellular proteinase and nuclease to produce DNA fragmentation. As a result of this unique mechanism of DNA breakage, NK314 showed substantial growth inhibition of topoisomerase II inhibitor-resistant tumors. Synonyms: NK-314; NK 314. Grades: 98%. CAS No. 208237-49-4. Molecular formula: C22H18ClNO4. Mole weight: 395.84. | |
| NLS-Cas9-D10A Nickase | NLS-Cas9-D10A Nickase. Nls-cas9-d10a nickase is a mutation form of cas9 nuclease which makes one active domain deactivated, thus it can only cut one single strand dna that is complementary to the guide-rna, producing one single strand cut. combined with two different grna, nls-cas9-d10a nickase produces two cut sites respectively and causes a double strand break. compared with the wild type cas9 nuclease, the two-grna guided cleavage can significantly reduce the off target effects. Group: Cloning Enzymes. Purity: 10μg; 50μg. Storage: Store at -20 ?. Source: E.coli. Pack: 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.4 at 25 °C. Cat No: CE-3505. | |
| PFM 01 | PFM 01, a cell-permeable N-alkylated Mirin derivative, is a nuclease-specific MRE11 inhibitor, which enhances non-homologous end-joining (NHEJ) and reduces homologous recombination. Synonyms: (5Z)-5-[(4-Hydroxyphenyl)methylene]-3-(2-methylpropyl)-2-thioxo-4-thiazolidinone; PFM01; PFM-01; PFM 01. Grades: ≥98% by HPLC. CAS No. 1558598-41-6. Molecular formula: C14H15NO2S2. Mole weight: 293.4. | |
| Proteinase K from Tritirachium album limber, Recombinant | Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments:pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. Group: Enzymes. Synonyms: Proteinase K; EC 3.4.21.64; Tritirachium alkaline proteinase; Tritirachium album serine proteinase; Tritirachium album proteinase K; endopeptidase K; 39450-01-6; protease K. CAS No. 39450-01-6. Purity: > 95%. Proteinase K. Activity: > 30U/mg. Storage: 4°C~-20°C. Form: Powder. Source: Pichia pastoris. Species: Tritirachium album limber. Proteinase K; EC 3.4.21.64; Tritirachium alkaline proteinase; Tritirachium album serine proteinase; Tritirachium album proteinase K; endopeptidase K; 39450-01-6; protease K. Cat No: NATE-1240. | |
| pTp | pTp is a competitive inhibitor of staphylococcal nuclease. Synonyms: Thymidine- 3', 5'- O- bisphosphate, sodium salt. Grades: ≥ 95% by HPLC. CAS No. 2863-4-9. Molecular formula: C10H16N2O11P2. Mole weight: 402.2. | |
| sgRNA Screening Kit | sgRNA Screening Kit. Sgrna screening kit provides a simple, reliable, and rapid method for assessing sgrna efficiency before cell transduction, allowing you to identify the highly effective crispr sgrna. cas9 nuclease nls is an rna-guided endonuclease that catalyzes site-specific cleavage of double stranded dna. the location of the break is within the target sequence 3 bases from the ngg pam (protospacer adjacent motif). the design of single guide rna (sgrna) is dependent on the target region close to the pam site. even if you pick a target sequence that fulfills all of the described requirements, sgrna specificity and activity is unpredictable. therefore, it is often recommended that multiple, different sgrnas be designed to target a gene of interest. components. gencrispr cas9 nuclease. 10x reaction buffer. positive control sgrna. positive control substrate. rnase-free water. Group: Cloning Enzymes. Purity: 30 reactions/kit. Storage: Store at -20 ?. Source: E.coli. Pack: 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol PH 7.4, at 25°C. Cat No: CE-3509. | |
| sgRNA Synthesis Kit | sgRNA Synthesis Kit. This product is designed to synthesize grnas in vitro. in the bacterial crispr/cas9 system, the cas9 nuclease associates with two rnas, the crispr rna (crrna) and the trans-activating crrna (tracrrna), to direct sequence-specific dna cleavage. the grna (guide rna) is a fusion of the natural crrna and tracrrna components. it contains an 18-20 base variable sequence that can be changed to target any dna sequence that is adjacent to an ngg proto-spacer adjacent motif (pam) on the 3 end of the target sequence. the sgrna synthesis kit allows user to fuse their choice of 18-20 base target sequence within the grna dna sequence provided, by pcr fusion. the kit also provides materials for in-vitro transcription of the generated grna dna in order to generate grnas which can be directly used for in-vivo genome editing. Group: Cloning Enzymes. Purity: 20 reactions/kit. Storage: Store at -20 ?. Source: E.coli. Pack: 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol PH 7.4, at 25°C. Cat No: CE-3510. | |
| site-specific DNA-methyltransferase (adenine-specific) | This is a large group of enzymes, most of which form so-called 'restriction-modification systems' with nucleases that possess similar site specificity [the nucleases are listed as either EC 3.1.21.3 (type 1 site-specific deoxyribonuclease), EC 3.1.21.4 (type II site-specific deoxyribonuclease) or EC 3.1.21.5 (type III site-specific deoxyribonuclease)]. A complete listing of all of these enzymes has been produced by R.J. Roberts and is available on-line at http://rebase. neb. com/rebase/rebase. html. Group: Enzymes. Synonyms: modification methylase; restriction-modification system. Enzyme Commission Number: EC 2.1.1.72. CAS No. 69553-52-2. Storage: Store it at +4 ?C for short term. For long term storage, store it at -20 ?C?-80 ?C. Form: Liquid or lyophilized powder. EXWM-1974; site-specific DNA-methyltransferase (adenine-specific); EC 2.1.1.72; 69553-52-2; modification methylase; restriction-modification system. Cat No: EXWM-1974. | |
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